Papers – Page 42 – TechConnect Briefs

Electrode Shapes for Electrowetting Arrays

Lienemann J., Greiner A., Korvink J.G., Albert Ludwig University, DE

We calculate the potential energy of an electrowetting array for different shapes and sizes of interdigital edge structures. To understand the dynamic effects at the beginning of the droplet motion is crucial for the optimization [...]

Low Energy Conformations of a Three-Helix Peptide in a All-Atom Biomolecular Forcefield

Herges Th., Schug A., Wenzel W., Forschungszentrum Karlsruhe, DE

Protein structure prediction remains one of the main outstanding challenges of theoretical biophysical chemistry. Its goal is to predict the fully three-dimensional tertiary structure of the protein on the basis of its amino acid sequence [...]

Highly Integrated Transparent Microchannel Chips for Continuous-flow PCR

Sun K., Matsuo S., Misawa H., Satellite Venture Business Laboratory, The University of Tokushima, JP

A microchannel chip for continuous-flow polymerase chain reaction (PCR) was developed using transparent materials. The microchannel was fabricated on a quartz glass substrate using standard photolithography and wet-etching techniques, and was sealed by another quartz [...]

A Homogenous Small Molecule Microarray for Enzymatic Assays

Ma H., Wang Y., Pomaybo A.S., Tsai C., Schmidt S.P., Morewood Molecular Sciences, Inc., US

Small molecule microarray as an emerging technology promises to play a pivotal role in chemical genetics. However, in spite of the fast development of this technology special requirements, such as the immobilization of small molecules [...]

Assembly of Oriented Virus Arrays by Chemo-Selective Ligation Methods and Nanolithography Techniques

Camarero J.A., Cheung C-L, Lin T., Johnson J.E., Weeks B.L., Noy A., De Yoreo J.J., Lawrence Livermore National Laboratory, US

Many experimental techniques in biology and biophysics, and applications in diagnosis and drug discovery, require proteins immobilized on solid substrates. In fact, the concept of arrays of proteins attached to a solid support has attracted [...]

Immunosensing Using Fabricated protein Microarrays

Howell S.W., Inerowicz H.D., Guirl L., Regnier F., Reifenberger R., Purdue University, US

Patterned microarrays of antibodies to specific bacteria were used to perform a series of bacterial immunoassays of E. Coli 0157:H7 and Renibacterium Salmoninarum (RS). Microarrays were fabricated using microcontact printing (MCP) and characterized using scanning [...]

Microfluidic System For High-Throughput Proteomics

Le Gac S., Arscott S., Druon C., Tabourier P., Rolando C., USTL, FR

The comparative study of a cell type whole proteome in normal, pathological conditions or under the administration of drugs, which is called differential proteomics, appears as a keytool to understand diseases. To reach this goal, [...]

Microfluidic Technology Applied to Protein Sizing and Quantitation

Barthmaier P., Agilent Technologies, US

Although protein analysis technologies are developing fast, the current standard method is still denaturing gel electrophoresis (SDS-PAGE). Many researchers rely on this traditional method that has not substantially changed in the past 30 years. However, [...]

Particle Movement in Microelectrode Structures in AC Electric Fields, Under the Influence of Dielectrophoresis and Electrohydrodynamics

Green N.G., University of Glasgow, UK

The use of AC electric fields for the manipulation, characterisation and separation of particles in suspension (dielectrophoresis: DEP) has become an area of considerable interest, both in basic research of the underlying physical mechanisms [1] [...]

Glucose Sensor Based On Enzyme Entrapment In Polyacrylamide Microgels

Lopez-Cabarcos E., Retama B.R., Mecerreyes D., Lopez-Ruiz B., Universidad Complutense, ES

Glucose oxidase (GOx), was encapsulated in polyacrylamide microgels with the aim to preserve the enzymatic activity and to fabricate a biosensor with these microgel particles (1-3). The biosensor presents great stability, but when it is [...]