Biomaterials – Page 49 – TechConnect Briefs

Design of a lipid nanovesicle system encapsulating bacteriophages integrated in a multiple emulsion formulation: a proof-of-concept

Balcão V.M., Azevedo A.F., Castro L.M., Costa C.I., Santos S., Matos C.M., Moutinho C.G., Teixeira J.A., Azeredo J.C., University Fernando Pessoa and Institute for Biotechnology and Bioengineering, PT

of the optimized bacteriophage-encasing nanovesicles encompassed determination of particle hydrodynamic size, size distribution and particle charge via DLS, surface morphology via CRYO-SEM, and thermal analysis via DSC, whereas antimicrobial activity of the nanoemulsions produced was [...]

Chromatographic Purification of Bacteriophages for Improved Surface Capture of Bacteria – Towards a General Approach

Naidoo R., Singh A., Glass N., Arya S., Evoy S., National Institute for Nanotechnology, University of Alberta, CA

We report in this study the largest surface capture density of E Coli using the wild-type T4 bacteriophage; whereby higher surface capture density can enable higher sensitivities for surface-based biosensors. This was possible due to [...]

Imaging Molecules and cells

Sharma R., Florida State University and TCC, US

Proteins provide the building blocks for multicomponent molecular units, or pathways, from which higher cellular functions emerge. These units consist of either assemblies of physically interacting proteins or dispersed biochemical activities connected by rapidly diffusing [...]

Nanoparticles that facilitate imaging of biological tissue and methods of forming the same

Sharma R., Florida State University, US

A superparamagnetic nanoparticle (SPIOM) containing antimyoglobin coated and polyethylene encapsulated biotin-avidin-iron-oxide core in center may be provided according to an example in the invention. These nanoparticles may be used as imaging contrast agents due to [...]

Creation of Protein Nanoarrays using Dip-Pen Nanolithography

Thompson D.G., McKenna E., Pitt A., Graham D., University of Strathclyde, UK

This presentation describes the creation of protein nanoarrays on a nitrocellulose surface using Dip-Pen Nanolithography and their use in studying protein:protein interactions in an assay format.

3D Molecular Theory of Solvation Coupled with MD for Nanomedical Sciences

Kovalenko A., National Institute for Nanotechnology, CA

We have coupled the statistical-mechanical, molecular theory of solvation (a.k.a. 3D-RISM-KH) with molecular dynamics (MD) simulation in the Amber molecular dynamics package to contract solvent degrees of freedom. This included a number of accelerating schemes [...]

3D-RISM-KH approach for biomolecular modeling at nanoscale: Thermodynamics of fibril formation and beyond

Blinov N., Dorosh L., Wishart D., Kovalenko A., University of Alberta/National Institute for Nanotechnology, CA

As an alternative to the conventional solvation models, we propose to use the three-dimensional molecular theory of solvation (3D-RISM-KH approach) as an essential part of a multiscale approach for nanomedical applications. Based on the rigorous [...]

Plasmonic Nanobubbles: tunable and transient probes for cancer diagnosis, therapy and theranostics

Lukianova-Hleb E., Drezek R., Hafner J., Lapotko D., Rice University, US

We report a tunable theranostic probe that can support the diagnosis, therapy and therapy guidance in one process in individual cancer cells. This probe is a plasmonic nanobubble (PNB), which is not a particle but [...]

Multi-Modal Superparamagnetic Nanoprobe: Harnessing Magnetic, Nuclear, and Optical Power for Diagnostic Imaging Applications

Bumb A., Regino C.A.S., Bernardo M., Dobson P.J., Choyke P., Brechbiel M.W., National Cancer Institute, US

Accurate characterization of the first draining sentinel lymph nodes in primary tumors is a challenging task where current methods rely on nodal size as the primary yardstick for differentiating benign from malignant nodes. Other parameters [...]

Qdot® nanocrystals in intracellular flow: ZAP-70, KI-67 and five-colors fluorophores panel

Sotnikov I., Jaron S., Konopleva M., O’Brien S., Andreeff M., Hildebrant J., Manis J., Brown J., Vorobjev I., Barteneva N., Immune Disease Institute, Harvard Medical School, US

We present, for the first time, the methodological approach for the measurement of cytoplasmic Zap-70 and nuclear Ki-67 proteins with Qdot® nanocrystals-labeled antibodies. Mononuclear cells from chronic lymphoid leukemia patients were used to detect Zap-70, [...]