Qdot® nanocrystals in intracellular flow: ZAP-70, KI-67 and five-colors fluorophores panel
Sotnikov I., Jaron S., Konopleva M., O’Brien S., Andreeff M., Hildebrant J., Manis J., Brown J., Vorobjev I., Barteneva N., Immune Disease Institute, Harvard Medical School, US
We present, for the first time, the methodological approach for the measurement of cytoplasmic Zap-70 and nuclear Ki-67 proteins with Qdot® nanocrystals-labeled antibodies. Mononuclear cells from chronic lymphoid leukemia patients were used to detect Zap-70, [...]