Ion channels are proteins that act as pores in cell membranes and allow only specific ions to pass through the cell wall. Here, we present a method for microfabrication of apertures in a silicon substrate using well-known cleanroom technologies. Apertures were etched through a silicon wafer using a plasma-etcher and then rendered hydrophobic with chemical vapor deposition of a Teflon surface layer. Characteristic measurements showing physiological single channel gating behavior of OmpF porin and alpha-hemolysin channel proteins have been made.
Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 8, 2005
Pages: 504 - 507
Industry sectors: Advanced Materials & Manufacturing | Sensors, MEMS, Electronics
Topic: MEMS & NEMS Devices, Modeling & Applications