Comparing Cytotoxicity of ZnO Nanoparticles in Exposure to Serum/Serum-free Cell Culture Medium

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The aim of this study is to evaluate the influence of serum in cell culture medium on nanotoxicity to human lung epithelial cells (A549). We found nano-ZnO had greater toxicity as it was suspended by serum-free medium, which could be attributed to serious agglomeration,rapid settlement of nanoparticles and starved cells. Nano-ZnO in 5% and 10% serum-contained medium had the same stable suspensions, and yet caused different toxicological responses in MTT and IL-8 assay. Though nanoparticles in serum-contained medium was stable, they still did not approach the real size distribution in air, which was monitored by Electrical Low Pressure Impactor (ELPI). In conclusion, serum-contained medium might be an appropriate environment to assess cytotoxicity of nano-ZnO, while its dispersibility still needed to be optimized and the content of serum should be regulated.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 21, 2010
Pages: 557 - 560
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topics: Environmental Health & Safety of Nanomaterials
ISBN: 978-1-4398-3415-2