transfection – TechConnect Briefs

Cationic lipid nanoemulsions for RNAi screening

Bruniaux J., Sulpice E., Mittler F., Texier I., Gidrol X., Navarro F.P., CEA/LETI/MINATEC, FR

Since the discovery of RNA interference, many efforts have been made to broaden perspectives of small interfering RNA (siRNA) in research and clinical work. siRNA shows a specific and effective gene silencing activity through a [...]

Synthesis and characterization of methoxy poly(ethylene glycol)-O-chitosan-polyethylenimine as a noviral carrier for gene delivery and expression

Yu Y.Y., Wang G., Wang Z., Cai L., Wang G., Wang Z., Yang X., Xu X.H., Xu Z.G., Wan X.P., Wang G., Wang Z., Xu X.H., Xu Z.G., Li Y., Jiang Q., Gao R., Sichuan University, CN

A novel water-soluble chitosan (CS) derivative，methoxy poly(ethylene glycol)-O-chitosan- polyethylenimine(mPEG-O-CS-PEI), was synthesized by grafting polycationic polyethylenimine (PEI) and methoxy poly (ethylene glycol) (mPEG) onto chitosan, the copolymer was characterized and confirmed by 1H-NMR and FT-IR spectra. [...]

Magnetically associated carbon nanotubes with mammalian cells: flow cytometry characterization and applications

Cai D., Blair D., Dufort F.J., Gumina M.R., Huang Z., Hong G., Kempa K., Ren Z.F., Chiles T.C., Boston College, US

Magnetic CNT can be driven into cells because it has an embedded Ni nanoparticle in its tip. We show herein that CNT-cell complexes are formed in the presence of a magnetic field. The complexes were [...]

Superparamagnetic Iron Oxide Nanoparticles for Multiple Biomedical Applications

Hofmann H., Hofmann M., Petri-Fink A., Steitz B., von Rechenberg B., Hofmann H., Hofmann M., Juillerat J., Swiss Federal Institute of Technology Lausanne, CH

Superparamagneticiron oxide nanoparticles (SPION) were mixed at various ratios with different polymer solutions to obtain SPION coated with PVA modified by functional groups (amino-SPION, carboxy-SPION, and thiol-SPION). Studying human melanoma cells by theses different functionalized [...]