proteins – Page 2 – TechConnect Briefs

Polymer Nanospheres for Improved Drug Delivery of Protein Therapeutics and Viral Antigens

Castor T.P., Aphios Corporation, US

Biologically adherent polymeric microsphere carriers have been shown to be effective in the oral delivery of proteins such as insulin and DNA plasmids. These microspheres are comprised of hydrophobic copolymers that appear to have a [...]

Transport of Ionic Electrolytes and Proteins through Semipermeable Membrane

Leung S.W., Trigo C., Daniels C.K., Bhushan A., Lai J.C.K., Idaho State University, US

Surfactants are known to influence functions of many proteins in membranes, cells and tissues. Because most studies to elucidate the effects of surfactants on membrane-bound proteins and cells employed heterogeneous or complex systems, it is [...]

Electric Field Process for the Fabrication of Higher Order Structures form Biomolecule Derivatized Nanoparticles

Heller M.J., Dehlinger D., Sullivan B., Esener S., University of California San Diego, US

An electronic microarray has been used to carry out directed self-assembly of higher order 3D structures from Biotin/Streptavidin and DNA derivatized nanoparticles. Structures with up to fifty layers of alternating biotin and streptavidin and DNA [...]

Liquid Crystal Interactions with Nanostructure and Applications to Diagnostics and Medical Research

Israel B.A., Murphy C.J., Abbott N.L., Platypus Technologies, US

We will present data demonstrating the use of liquid crystal detection of protein in the development of rapid, label free diagnostics and assays. We control the orientation of liquid crystals by the fabrication of surfaces [...]

Conical Tomograph: A Method for the Study of Macromolecular Assemblies

Zampighi G.A., Zampighi L., Fain N., Lanvazecchia S., Zampighi G.A., Zampighi L., University of California, Los Angeles School of Medicine, US

We have developed conical tomography to study the structure of macromolecular assemblies and organelles in their native cellular environment. Unlike the other related types of tomography, conical tomography has a higher throughput and more importantly, [...]

A Monte Carlo Study of a Model of Globular Protein Nanoparticles

Pagan D.L., Gracheva M.E., Gunton J.D., Lehigh University, US

We explore the metastable fluid-fluid coexistence curve of the modified Lennard-Jones model of globular proteins of ten Wolde and Frenkel [4]. Using grand canonical Monte Carlo simulations along with mixed-field finitesize scaling and histogram rewighting, [...]

Structure Based Design of Protein Ligands: A Study of Antibody-like Scaffolds Targeted Against the Anthrax Toxin

Shiflett P., Hong-Geller E., Allen D., Lauer S., Lehnert B., Lehnert N., Nolan J., Lehnert B., Lehnert N., Gupta G., Los Alamos National Laboratories, US

We have adopted structure-based approaches to enhance the affinities of two single chain antibodies, scFv1 and scFv4, that bind to two different epitopes on the Protective Antigen (PA), a toxin from Bacillus anthracis. In one [...]