Landscape phage – TechConnect Briefs

Precise Phage-navigating Metastatic Breast Cancer-targeted Paclitaxel-Micell Nanomedicines

Petrenko V.A., Torchilin V.P., Auburn University, US

Breast cancer is one of the most common cancers in the USA. Despite the increase of relative survival rate of women with breast cancer for the last decades, it is still low (~26%, 5 years) [...]

Self-navigating drug delivery nanovehicles driven by polyvalent multifunctional phages and their promiscuous proteins

Petrenko V.A., Gillespie J.W., Auburn University, US

Development of precision medicines acting specifically at the site of disease has been a long sought goal for the last century, starting from pioneering works of Paul Ehrlich, who suggested the concept of site-directed chemotherapeutics [...]

Landscape phage probes for PC3 prostate carcinoma cells

Jayanna P.K., Deinnocentes P., Bird R.C., Petrenko V.A., Auburn University, US

Cancer chemotherapy is complicated by a lack of selective drug targets. The use of liposomes as vectors for drug delivery has provided a route to selective accumulation of nanoparticles in the tumor interstitium due to [...]

Modulating affinity of landscape phage nanoparticles by mutagenesis of the major coat protein

Kuzmicheva G.A., Eroshkin A.M., Potemkin V.A., Petrenko V.A., Auburn University, US

Phage peptide libraries proved to be invaluable source of the ligands specifically binding numerous analytes. Diversity and repertoir of the libraries are critical for the successful selection of ligands. In landscape libraries guest peptides are [...]

Phage-Derived Bioselective Nanovehicles for Drug and Gene Delivery

Petrenko V.A., Auburn University, US

Phage display technology rests on two fundamental concepts: combinatorial chemistry and fusion phage. We first demonstrated fusion of the major coat protein of the phage with foreign random peptides, which results in landscape phage libraries. [...]

Nanofabrication of Bioselective Materials using Diverse Nanolandscapes Displayed on Live Viruses

Petrenko V.A., Olsen E.V., Sykora J.C., Kouzmitcheva G.A., Sorokulova I.B., Brigati J.R., Chen I-H., Chin B.A., Vodyanoy V.J., Auburn University, US

Filamentous phage, such as fd used in this study, are thread-shaped bacterial viruses. Their outer coat is a tube formed by thousands equal copies of the major coat protein pVIII (Figure 1, left). We constructed [...]