electroporation – TechConnect Briefs

Dependence of the Energy of Electric Pulse Required to Electroporate the Cell on the Pulse Duration for Pulses from 50 ns to 500 ms

Saulis G., Bitinaitė A., Vytautas Magnus University, LT

To successfully implement the technology of food processing with pulsed electric fields (PEF), it is important to know what particular electric treatment (pulse duration, field strength, etc.) would require the minimum energy. Here, theoretical relationships [...]

Estimation of the Energetic Barrier to Pore Formation and the Pore Radius Corresponding to this Barrier for Different Cell Lines

Bitinaitė A., Saulis G., Vytautas Magnus University, LT

The permeability of the cell membrane can be modified by exposing of cells to high-voltage electric pulses leading to the formation of nanometer-sized pores in the cell membrane (electroporation or nanoporation). This phenomenon is widely [...]

Cellular Delivery Modalities for Semiconductor Quantum Dots: A Comparative Analysis

Field L.D., Naval Research Lab/ University of Maryland College Park, US

Fluorescence continues to be one of the most useful tools employed by life scientists to visualize cellular processes. Semiconductor nanocrystals or quantum dots (QDs) have been demonstrated to be superior alternatives to traditional fluorophores (dyes [...]

Fabrication of electrical microfluidic chip for electroporation of mammalian cells

Shahini M., Wijngaarden F.V., Yeow J.T.W., University of Waterloo, CA

The point of this paper is to demonstrate the use of a quick and cheap fabrication method to realize a laser-ablated microfluidic channel for single cell electroporation. Traditional lithography of microchannel with electrode in MEMS [...]

Analysis of the Relationships Between the Parameters of the Electric Pulse

Saulis G., Vytautas Magnus University, LT

Theoretical relationships between the parameters of the electric pulse which is necessary for the poration of the cell have been obtained. Analysis has been made for the square wave, exponential and sine wave ac electric [...]

Combining gene therapy and nanomedicine to enhance the therapeutic potential of T cells

Choi Y., Torikai H., Lee D.A., Lee D.Y., Li K.C., Li Z., Lo D., Qin G., Tung C.H., Tasciotti E., Raphael R., Biswal S.L., Ang S., Lee D.A., Lee D.Y., Li K.C., Li Z., Cooper L.J.N., M.D. Anderson Cancer Center, US

we have developed a high throughput electroporator (HiTE) to manipulate large numbers (~10^9) of T cells ex vivo for clinical uses. HiTE is designed to electro-transfer RNA species to redirect T-cell specificity and gold nanoparticles [...]

Size of the Pores Created by an Electric Pulse: Microsecond vs Millisecond Pulses

Saulis G., Saule R., Vytautas Magnus University, LT

Modeling studies have revealed that the pulse of nanosecond-duration should create smaller pores than the pulse of micro-millisecond duration. It can be expected, that similar differences should also exist between the pore populations created by [...]

In Situ Investigation of Uptake Phenomena of Biological Molecules and Silica Nanoparticles into Mammalian Cells in Microstructures

Lee W.G., Bang H., Yun H., Kim J.A., Cho K., Shin Y.S., Chung C., Chung N.C., Chung C., Chung N.C., Chang J.K., Han D-C, Seoul National University, KR

Electroporation is a powerful gene transfection method to deliver foreign biological molecules such as DNA and protein into living cells with electric pulsations. However, conventional cuvette-type electroporation techniques generally suffer from poor transfection efficiency and [...]

Highly Efficient Electro-permeabilization of Mammalian Cells Using Micro-electroporation Chip

Shin Y.S., Kim J.K., Lim S.H., Lee Y., Lee Y.K., Cho K., Lee Y., Lee Y.K., Chung C., Chung S., Chung C., Chung S., Han D-C, Chang J.K., Seoul National University, KR

Electroporation, is widely used method that can be directly applied to the field of gene therapy. However, very little is known about the basic mechanisms of DNA transfer and cell response to the electric pulse. [...]

Electrodynamic Transport, Electroporation and Lysis of Cells in Pharmacological and Bioanalytical Microsystems

Przekwas A.J., Chen Z.J., Athavale M., CFD Research Corporation, US

Biological information is stored in cells and is the prime object of interest of pharmacological, biomedical, and bioanalytical professionals. Whatever the interest: elcectroporative drug delivery and pharmacodynamics, in vivo PCR, infection pathology, DN