DNA – Page 6 – TechConnect Briefs

Rapid DNA Sequence Identification based on Electrostatic Interactions with Unmodified Gold Nanoparticles

Li H.X., Rothberg L.J., University of Rochester, US

Sequence-specific detection of DNA play more and more important role in clinical diagnosis and molecular biology research. Most assays identify specific sequence through hybridization of an immobilized probe to the target analyte after the latter [...]

Microcantilever Array Sensor for DNA and RNA Detection

Cherian S., Natesan M., Macis E., Cain R., Tedeschi L., Citti L., Palchetti I., Tombelli S., Raiteri R., University of Genova, IT

A microcantilever array platform is used for the detection of DNA hybridization and mRNA using selected immobilized oligonucleotides. Parallel detection on sixteen cantilevers are carried out simultaneously. The immobilization procedure and the results of the [...]

Synthesis of new Metal Complex – Biomolecule Hybrids as Redox Sensors for Specific Nucleobases

Gasser G., Bond A.M., Graham B., Kosowski Z., Spiccia L., Monash University, AU

There are a number of redox-active supramolecular receptors described in the literature, but those containing ferrocene as a redox-active reporter group are the most frequently cited. It has been shown that modified ferrocenyl conjugates can [...]

Boolean Networks (BN) Based Molecular Biology Modeling: Protein Process

Yeol J.W., Barjis I., Barjis J., Barjis I., Barjis J., Berri S., Ryu Y., Polytechnic University, US

In this paper, we introduce a new application of modeling tool; Boolean Networks (BN). In particular, it shows how, by means of a Boolean Networks (BN), the protein production process can be modeled and analyzed. [...]

Imaging Ellipsometry: A Powerful Method for Biochip Development, QC and Evaluation

Laging M., Hoenig D., Eing A., Accurion, US

Biochip applications nowadays are taking the step from DNA to protein applications. Proteins show a vasting variety concerning their individual chemical behaviour, demanding new techniques regarding chip substrates and immobilization chemistries. Furthermore the use fluorescent [...]

Nanotechnology Based on Spatially Fixed ds DNA (RNA) Molecules

Zakharov M.A., Yevdokimov Yu.M., Skuridin S.G., Nechipurenko Yu.D., Engelhardt Institute of Molecular Biology of RAS, RU

A new strategy of creating nucleic acid nanostructures is described. Our approach is based not on isotropic dsDNA (or RNA) molecules, but on these molecules fixed in spatial structure of their liquid-crystalline particles.The nanostructure formed [...]

Multivalent Circular Aptamers: Versatile Nanostructures for Biomedical Applications

King G.C., Knox S.M., Williams B.B., Harris J., Gupta D.M., Di Giusto D.A., Gabutero E.R., Tyrelle G.D., Lai Y.-C., Aung M.T., McCutcheon S.T., Voelcker N., Le H.T.T., School of Biotechnology and Biomolecular Sciences, AU

Much recent interest has focussed on applications of DNA in nanotechnology, while chemical biologists have adapted evolutionary processes to generate aptamers, folded nucleic acids with molecular recognition properties similar to protein antibodies. As yet there [...]

Structural Characterization of Novel Micro- And Nano-Scale Non-Viral DNA Delivery Systems for Cutaneous Gene Therapy

Foldvari M., Badea I., Wettig S., Verrall R., Bagonluri M., University of Saskatchewan, CA

Our main goal is to develop micro/nano-scale self-assembling delivery vehicles for cutaneous gene therapy. The objective of this study was to characterize the structural and physicochemical properties of novel dicationic lipid-based DNA complexes by atomic [...]

A New Semiconductor-Wafer Market Based on the Deepening of Surface Undulations to Form Strongly Textured Atomic Ridges (STAR) With Pitches from 0.6 to 5.4 nm: Model Demonstrations in Electronics and the Physical and Life Sciences

Kendall D.L., Kendall M., Kendall D.L., Kendall M., StarMega Corp, US

A broad patent position has been established that produces semiconductor wafers containing physically-deepened grooves, ridges and quantum-dots on particular crystal planes with perfectly spaced pitches of 0.9 to 5.4 nm determined by the inherent crystal [...]

Sol-gel Silane Films for DNA Microarray Experiments

Saal K., Tätte T., Kink I., Kurg A., Lõhmus A., Lõhmus R., Mäeorg U., Rinken A., Lõhmus A., Lõhmus R., University of Tartu, EE

Aim of the current research is development of a method for producing a new kind of aminosilanized films suitable for applications utilizing immobilized DNA molecules. We report on new progress in a procedure proposed in [...]