aptamer – TechConnect Briefs

Analytical system capable of rapidly quantifying food-borne pathogen for food safety and public health

Khang J., Kim D., Chung K.W., Lee J-H., Luminescent MD, LLC, US

Cost-effective and easy-to-use biosensor was developed for the rapid quantification and monitoring of Escherichia (E.) Coli O157:H7 in sample using E. Coli O157:H7 aptamer, graphene oxide (GO)/iron nanocomposites, and guanine chemiluminescence detection. E. Coli O157:H7 [...]

Breast cancer diagnosis system using the combination of carcinoembryonic antigen aptamer, horseradish peroxidase-mimic hemin, and graphene sheet

Chong S., Cho K., Chong S., Khang H.J., Lee J-H., Lee S-Y, Kim Y.T., Lee J-H., Lee S-Y, Luminescent MD, LLC, US

In order to early diagnose breast cancer with the rapid quantification of carcinoembryonic antigen (CEA) in human serum, highly sensitive DNA aptasensor with 1,1-oxalyldiimidazole chemiluminescence (ODI-CL) detection was developed using the combination of CEA aptamer, [...]

Application of chemiluminescent resonance energy transfer and graphene sheet for the rapid quantification of mercury in a sample

Kwon M., Park Y., Lee J-H., Lee S-Y, Kim Y.T., Lee J-H., Lee S-Y, Luminescent MD, LLC, US

Using intra chemiluminescent resonance energy transfer (CRET) between DNA aptamer and fluorescein and physical property of graphene sheet, cost-effective and simple biosensor was developed for the rapid quantification of Hg2+ in a sample. The limit [...]

Fabrication of Aptamer-Functionalized Gold Nanorod Biosensor for Colorimetric Detection of Lysozyme

Tohidi Moghadam T., Ranjbar B., Tarbiat Modares University, IR

Gold nanorods (GNRs) have been nominated as good candidates for biosensing, diagnostics, and imaging technologies. The wavelength dependence of the longitudinal surface plasmon resonance of gold nanorods on minute changes of refractive indexes makes them [...]

Dual-aptamer-based delivery vehicle of doxorubicin to both PSMA (+) and PSMA (–) prostate cancers

Jo H., Ban C., Pohang University of Science and Technology, KR

We have designed a dual-aptamer complex specific to both prostate-specific membrane antigens (PSMA) (+) and (–) prostate cancer cells. In the complex, an A10 RNA aptamer targeting PSMA (+) cells ¬¬and a DUP-1 peptide aptamer [...]

Combination of magnetic and non-magnetic graphene oxide to develop chemiluminescent aptasensor capable of rapidly quantifying tumor markers

Choi H-K, Park L., Kim J., Lee J-H., Luminescent MD, LLC, US

Rapid and sensitive chemiluminescent aptasensor capable of rapidly sensing tumor markers in human serum with the combination of magnetic and non-magnetic graphene oxide. Human serum (150 µl) containing tumor markers (e.g., AFP and PSA) was [...]

Background free biosensor using the combination of graphene oxide and Fe3O4 nanoparticles

Cho S., Park L., Chung R., Lee J-H., Luminescent MD, LLC, US

Cost-effective and rapid biosensor with background free chemiluminescence (CL) detection was developed using ssDNA aptamer-conjugated 6-FAM fluorescein (DAF), graphene oxide bound with magnetic nanoparticle (GMN), and 3,4,5-trimethoxyphenylglyoxal (TMPG). When thrombin was incubated in TE buffer [...]

AS1411 aptamer-conjugated polymeric micelle for targetable cancer therapy

Lai P.S., Pai C.L., Hsu C.Y., National Chung Hsing University, TW

Targetable drug delivery system is a promising tool for cancer treatments. Herein we report a novel strategy of using G-quadruplex AS1411 aptamer-conjugated polyethylene-polylactide formed polymeric micelles (apt-PEG-PLA-PM) as a drug carrier to target cancer cells [...]

Creation of an Aptamer Nanobiosensor for Ultra-Sensitive Detection of Heart Failure

Darnaud M., Ricketts A., Graham D., University of Strathclyde, UK

This project focuses on discovering a new aptamer sequence that binds specifically and with high affinity to Brain Natriuretic Peptide (BNP), which is indicative of the early stages of heart failure. A library of random [...]