Petrenko V.A. – TechConnect Briefs

Precise Phage-navigating Metastatic Breast Cancer-targeted Paclitaxel-Micell Nanomedicines

Petrenko V.A., Torchilin V.P., Auburn University, US

Breast cancer is one of the most common cancers in the USA. Despite the increase of relative survival rate of women with breast cancer for the last decades, it is still low (~26%, 5 years) [...]

Self-navigating drug delivery nanovehicles driven by polyvalent multifunctional phages and their promiscuous proteins

Petrenko V.A., Gillespie J.W., Auburn University, US

Development of precision medicines acting specifically at the site of disease has been a long sought goal for the last century, starting from pioneering works of Paul Ehrlich, who suggested the concept of site-directed chemotherapeutics [...]

Molecular toolkits for engineering of self-navigating drug delivery vehicles

Gillespie J.W., Auburn University, US

Analysis of multi-thousand clone populations of the cancer cell-binding landscape phages and their multi-motif proteins using massively parallel sequencing techniques allowed the discovering of a variety of short motifs serving as elementary binding units during [...]

Optimization of Phage-Based Magnetoelastic Biosensor Performance

Huang S., Yang H., Johnson M., Wan J., Chen I., Petrenko V.A., Chin B.A., Auburn University, US

A magnetoelastic (ME) platform coated with a bio-molecular recognition element (bacteriophage) for selective and specific recognition of Bacillus anthracis spores is described. ME materials have a mass-sensitive, characteristic, resonant frequency. In response to the binding [...]

Optimization of Phage-Based Magnetoelastic Biosensor Performance

Huang S., Yang H., Johnson M., Wan J., Chen I., Petrenko V.A., Chin B.A., Auburn University, US

A magnetoelastic (ME) platform coated with a bio-molecular recognition element (bacteriophage) for selective and specific recognition of Bacillus anthracis spores is described. ME materials have a mass-sensitive, characteristic, resonant frequency. In response to the binding [...]

Landscape Phage Probes for Breast Cancer Cells

Fagbohun O.A., Bedi D., Jayanna P.K., Deinnocentes P.A., Bird R.C., Petrenko V.A., Auburn University, US

Landscape phage libraries are collections of filamentous phages displaying random foreign peptides on all 4,000 surface domains of the major coat protein. They serve as a rich source of bioselective materials that are used in [...]

Landscape phage probes for PC3 prostate carcinoma cells

Jayanna P.K., Deinnocentes P., Bird R.C., Petrenko V.A., Auburn University, US

Cancer chemotherapy is complicated by a lack of selective drug targets. The use of liposomes as vectors for drug delivery has provided a route to selective accumulation of nanoparticles in the tumor interstitium due to [...]

Landscape phage libraries as a source of bioselective nanomaterials

Kuzmicheva G.A., Sorokulova I.B., Jayanna P.K., Petrenko V.A., Auburn University, US

New generations of targeted diagnostic, therapeutic and imaging nanodevices require robust molecular recognition interfaces, which bind their targets with high specificity and selectivity. These bioselective interfaces can be formed by recombinant filamentous phages or their [...]

Modulating affinity of landscape phage nanoparticles by mutagenesis of the major coat protein

Kuzmicheva G.A., Eroshkin A.M., Potemkin V.A., Petrenko V.A., Auburn University, US

Phage peptide libraries proved to be invaluable source of the ligands specifically binding numerous analytes. Diversity and repertoir of the libraries are critical for the successful selection of ligands. In landscape libraries guest peptides are [...]

Phage-Derived Bioselective Nanovehicles for Drug and Gene Delivery

Petrenko V.A., Auburn University, US

Phage display technology rests on two fundamental concepts: combinatorial chemistry and fusion phage. We first demonstrated fusion of the major coat protein of the phage with foreign random peptides, which results in landscape phage libraries. [...]