US – Page 377 – TechConnect Briefs

Label Free Colorimetric Detection of Single Nucleotide Polymorphisms in Pcr Amplified Genomic DNA

Li H.X., Rothberg L.J., University of Rochester, US

Analysis of DNA is increasingly important in clinical diagnosis, pathology and genetics1, 2. Single nucleotide polymorphisms (SNPs) in genomic DNA are known to be responsible for a number of hereditary conditions3 and cancers4. Nearly all [...]

Rapid DNA Sequence Identification based on Electrostatic Interactions with Unmodified Gold Nanoparticles

Li H.X., Rothberg L.J., University of Rochester, US

Sequence-specific detection of DNA play more and more important role in clinical diagnosis and molecular biology research. Most assays identify specific sequence through hybridization of an immobilized probe to the target analyte after the latter [...]

Microchannel Immunoassays with Electrochemical Detection in Devices Constructed from Low Temperature Co-fired Ceramic and Screen Printed Gold

Fakunle E.S.O., Fritsch I., University of Arkansas, US

We report the first self-contained electrochemical enzyme linked immunosorbent assay in microchannel devices constructed from low temperature co-fired ceramic (LTCC) with screen-printed gold for fast and sensitive assays. No one has previously reported a device [...]

Utilizing Nanoscale Patterned Proteins to Create Neuronal Cells Circuits

Wang W., Chun C-J, Gregory C., Narayanan S., Poeta M., Hickman J.J., University of Centra Florida, US

Micro-patterning of neuronal cells in vitro is a critical step for studies in the fundamental biology of neuron-neuron and neuron-surface interactions. The culturing of the neuronal cells on patterned self-assembled monolayers (SAMs), in some cases, [...]

Modeling Particle Hydrodynamic Transport in an Idealized Nanoscale Bio-Motor

Longest P.W., Pidaparti R.M., Virginia Commonwealth University, US

In the cell, hydrodynamic characteristics of the nuclear pore complex (NPC) are characterized by Stokesian liquid flow dynamics and high relative particle diffusion rates. As such, the hydrodynamics model of the NPC will be based [...]

Purification of PCR-Inhibitory Components by Cellulose Acetate Membranes

Mohamed H., Szarowski D.H., Lepak L.A., Spencer M.G., Martin D.L., Caggana M., Turner J.N., New York State Department of Health, US

Conventional methods for extracting DNA from blood results in the presence of heme, which is an inhibitory factor decreasing PCR sensitivity. Current techniques for removing the inhibitors are laborious, costly, and/or sample-specific. We investigated using [...]

A Novel Electrochemical Nitric Oxide Sensor: Aligned RuO2 Nanowires Deposited on Pt filament

Wang C.Y., Liu J., Piantadosi C.A., Allen B.W., Duke University, US

We fabricated a novel electrochemical NO sensor by depositing aligned ruthenium oxide (RuO2) nanowires on a fine Pt filament. This device exhibited a high current density and a low detection limit for NO in aqueous [...]

Nano-Cantilevers for an Ultra-Sensitive Bio-Assay

Zheng J., Klein K., Pucha R., Sitaraman S.K., Sitaraman S.V., Merlin D., Rajalakshmi S., Sarma D.S.R., Gerwirtz A., Sitaraman S.K., Sitaraman S.V., Georgia Institute of Technology, US

Micro and Nano-scale cantilever arrays are being developed for an ultra sensitive bio-assay (Figure 1). The advantage of these cantilevers is that they eliminate the use of laser for deflection detection, which is impractical for [...]

Nanobiodevices Integrating Biomolecular Motors

Ramachandran S., Wu D., Nitta T., Tucker R., Phillips A., Hess H., University of Washington, US

Biomolecular motors, such as the motor protein kinesin, can serve as force-generating modules in hybrid nanodevices. We will present recent progress in utilizing these motors for nanoscale transport of target reagents and discuss applications, such [...]

Surface Modification Chemistries and their Utilization in Microarrays, Diagnostics and Drug Delivery

Lodhi M.A., Opperman G.W., Wall J.V., Anderson A.B., SurModics, Inc., US

Availability of DNA sequence data on numerous organisms has revolutionized the way biological queries are addressed today. Focus of activity has largely shifted from discovery to application. Things that were considered far-fetched only a few [...]