Bio Nano Particles – TechConnect Briefs

Highly Selective Capture and Transport of Rare Cells in Microfluidic Channels

Ward M.D., Grodzinski P., Los Alamos National Laboratory, US

Collection of rare target cells in clinical samples such as disseminated tumor cells in peripheral blood or fetal cells in maternal blood poses difficult challenges to currently available cell separation methods. A novel, two stage [...]

Biomimetic Design of a Microfluidic Device for Auto-separation of Leukocytes from Whole Blood

Shevkoplyas S.S., Yoshida T., Munn L.L., Bitensky M.W., Boston University, US

Leukocytes comprise less than one percent of all blood cells. Enrichment of their number, starting from a sample of whole blood, is the required first step of many clinical and basic research assays. We created [...]

A Microfluidic System for Continuous Magnetophoretic Separation of Suspended Cells Using Their Native Magnetic Properties

Han K-H, Bruno Frazier A., Georgia Institute of Technology, US

This paper presents the characterization of a continuous magnetophoretic microseparator for separating white and red blood cells from whole blood by using a high gradient magnetic separation method and microfabrication technology. The magnetophoretic microseparator directly [...]

Strong DC and Low-Frequency AC Fields for the Manipulation of Particles and Fluids in Microfluidics

Kumar A., Qiu Z., Khusid B., Yeksel M., Acrivos A., New Jersey Institute of Technology, US

Electroosmosis and electrophoresis are considered to be the main phenomena for transport of aqueous liquids and particles in microfluidic devices because field-based techniques do not require any moving parts and can be incorporated more favorably [...]

Numerical Modelling of a Microfluidic Ultrasonic Particle Separator

Townsend R.J., Hill M., Harris N.R., White N.M., Beeby S.P., University of Southampton, UK

Particles held in a fluid suspension and within an acoustic standing wave experience an acoustic radiation force. The force causes particles to move to the pressure nodes of the acoustic field creating a concentrate, contributing [...]

Quantum Dot-Mediated Separation-Free Assay for Point Mutation Detection

Yeh H.C., Ho Y.P., Wang T.H., John's Hopkins University, US

We describe a novel semiconductor quantum dot (QD)-based biosensing assay for single nucleotide polymorphism (SNP) detection. By integrating a QD-mediated dual-color fluorescence coincidence detection method with oligonucleotide ligation assay (OLA), we have achieved rapid SNP [...]

Labeling and Imaging of Human Mesenchymal Stem Cells with Quantum Dot Bioconjugates during Proliferation and Osteogenic Differentiation in Long Term

Shah B., Clark P.A., Stroscio M., Mao J.J., University of Illinois at Chicago, US

Tissue engineering approaches have the potential to repair damaged bone using a patient’s own mesenchymal stem cells (hMSCs). The objective of this study was to determine the feasibility of long-term labeling of hMSCS with semiconductor [...]

Liposome Encapsulation of Fluorescent Nanoparticles: Quantum Dots and Silica Nanoparticles

Chen C-S, Yao J., Durst R.A., Cornell University, US

Quantum dots (QDs) and silica nanoparticles (SNs) are new classes of fluorescent probes that overcome the limitations encountered by organic fluorophores in bioassay and biological imaging applications. We encapsulated QDs and SNs into liposomes by [...]

Fluorescent Silica Beads for Detection of Cervical Cancer

Iyer S., Kievesky Ya., Woodworth C.D., Sokolov I., Clarkson University, US

We present the use of self-assembled fluorescent silica (glass) bids for detection of cervical cancer. The cells from three different individuals (3 normal and 3 tumor) were tested for affinity by using the bids, a [...]

Self-Assembly of Fluorescent Silica (glass) Beads

Kievesky Ya., Sokolov I., Clarkson University, US

We report self-assembly of fluorescent silica (glass) bids. The bids are a few microns glass nanoporous particles which contain florescent dyes imbedded in the pores. The pores are self-closed, so the dye does not leak [...]

Formation of Polymer Nano-particles in Supercritical Fluid Jets

Gokhale A.A., Khusid B., Dave R.N., Pfeffer R., New Jersey Institute of Technology, US

The supercritical antisolvent process is an emerging technology for the production of micro- and nanometer-sized particles for a wide variety of bio-medical pharmaceutical applications. In this method, an organic liquid solution of the compound of [...]

Water-Soluble, Carboxyl-Modified Silicon Nanoparticles

Lee S., Cho W-J, Korea Institute of Science and Technology, KR

Since silicon has both the well-known chemistry and the industrial benefits of non-toxic and low cost, an interest in Si nanoparticles has rapidly increased. We will discuss a practical surface modification of Si nanoparticles prepared [...]

Synthesis and Properties of Magnetic Nanoparticles with Potential Applications in Cancer Diagnostic

Neamtu J., Jitaru I., Malaeru T., Georgescu G., Kappel W., Alecu V.-V., National Institute for Research, RO

The size, shape and composition of magnetic nanoparticles are very attractive for diagnostic tools of cancer tumors and targeting treatment in HIV infection. The goal of this paper is the preparation from colloidal solutions of [...]

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