Papers – Page 29 – TechConnect Briefs

Enhancement of Osteobalstic Bone Cell Proliferation Incubated on Plasma Treated Polymers

Mahmood M., Fejleh P.M., Ishihara H., Karmakar A., UALR, US

It was determined that polyurethane polymers have the potential to be valuable biomaterials. Some major problems with this material involve its low biocompatibility and its inability to induce cellular adhesion and proliferation on its surface, [...]

Delivery of ferritin-encapsulated gold nanoparticles on desired surfaces

Zheng B., Uenuma M., Yamashita I., Uraoka Y., Nara Institute of Science and Technology, JP

A ferritin mutant protein, which can efficiently catch gold nanoparticles of various sizes and endow gold nanoparticles a new property to adsorb to Titanium/Titanium oxide surface, was reported.

Chitosan Membrane in Combinations with Nanoparticles and Adriamycin as a Treatment to Inhibit Glioma Growth and Migration

Leung S.W., Gao W., Gu H., Bhushan A., Lai J.C.K., Idaho State University, US

Our previous and on-going studies indicated that U87 cells cultured on chitosan film/membrane exhibited significantly slower growth and proliferation kinetics compared to U87 cells cultured in the absence of chitosan film/membrane. Chitosan exhibits anti-microbial activities [...]

Construction and properties of zein-based cell supports and scaffolds

Padua G.W., Wang Q., Wang Y., Wang Q., Wang Y., University of Illinois Urbana-Champaign, US

Zein, a protein abundant in corn, is capable of self-assembly and may form a variety of structural morphologies (Wang et al 2004, Wang et al 2008a). Zein micro-fibers (Figure 1.), films, and sponges (Figure 2) [...]

Gold nanoparticles cellular toxicity and recovery

Mironava T., Hadjiargyroy M., Rafailovich M.H., SUNY at Stony Brook, US

Gold nanoparticles (AuNPs) have unique electronic and chemical properties and hence are used in many applications. However, their potential health risk(s) and interactions with cells are not fully known. Most previous studies have focused determining [...]

A novel stem cell tag-less sorting method

Roda B., Lanzoni G., Alviano F., Zattoni A., Costa R., Di Carlo A., Marchionni C., Franchina M., Ricci F., Tazzari P., Pagliaro P., Scalinci S.Z., Bonsi L., Reschiglian P., Bagnara G.P., University of Bologna, IT

New methods for stem cells isolation are being developed. Common procedures for stem cell sorting involve flow cytometry which makes use of fluorescent or magnetic tags to isolate and separate cells which are phenotypically different. [...]

A tag-less method for the direct isolation of HUVEC (human umbilical vein endothelial cells) by gravitational field-flow fractionation as a starting point for gene expression analysis

Lattuada D., Roda B., Pignatari C., Magni R., Cattaneo A., Bolis G., Reschiglian P., University of Bologna, IT

HUVECs (human umbilical vein endothelial cells) are a model for prediction of endothelial dysfunction in various diseases. HUVECs are enzymatically removed from the umbilical vein by collagenase. This method allows for a good sample yield, [...]

A Novel Microscale Coulter Counter for Cell Monitoring and Detection

Wu Y., Critser J., Almasri M., University of Missouri, US

A MEMS based Coulter counter for cell detection and monitoring is designed, fabricated and characterized. The novelty of this design is the use of mixing, focusing and detection on a single chip as well as [...]

Analysis and characterization of multifunctional oligothiophene-silica hybrid nanomaterials by AF4-MALS-Fl

Rambaldi D.C., Zattoni A., Reschiglian P., Melucci M., Zambianchi M., Barbarella G., Bonacchi S., Montalti M., Rampazzo E., University of Bologna, IT

We present results indicating that Asymmetrical Flow Field-Flow Fractionation (AF4) on-line coupled with Multi-Angle Light Scattering (MALS) and Fluorescence (FD) detection can become the unparallel methodology for morphological and optical characterization of new, structured, fluorescent [...]

Hollow-fiber flow field-flow fractionation: a novel pre-MS method for proteomics

Rambaldi D., Zattoni A., Reschiglian P., Johann C., University of Bologna, IT

It is well acknowledged that both number and dynamic range of the proteins present in biological systems lead to the need of a “simplified” and more efficient way to proteome analysis. The availability of so-called [...]