Patel D., Zhang G., Sun X., Gobin A.M., Keynton R.S.
University of Louisville, US
Keywords: biomarkers, detection, gold nanoshells
The focus of this project is to improve the detection of antigen/biomarkers/viral vectors using gold nanoshells (GNS). The assay used in this project is based on aggregation of GNS in presence of antigen or viral vectors. GNS are coated with a Protein-G conjugated to thiol–poly(ethylene) glycol–amine and Goat anti-rabbit antibody. Our data shows that fully assembled nanoparticles in presence of antigen, rabbit IgG , allow detection at concentrations in the ng/ml to pg/ml. The lower detection limit of this improved assay was 0.5 pg/mL within 10 mins. This shows a 3 order of magnitude improvement over that previously reported.
Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Published: June 18, 2012
Pages: 17 - 20
Industry sector: Medical & Biotech
Topics: Diagnostics & Bioimaging, Sensors - Chemical, Physical & Bio
ISBN: 978-1-4665-6276-9