Towards an asynchronous magnetic bead rotation method for immunological assays

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Much attention has been focused on using magnetic particles for biodetection applications on chip. Most of these applications involve using the magnetic particles either for translational motion in a magnetic field, or as labels for magnetoresistive sensing. Here we present an application using paramagnetic particles as the basis for rotational motion in a magnetic field, correlating the rate of rotation of a magnetic cluster with the concentration of a sample target analyte. In a rotating magnetic field, paramagnetic particles rotate out of synch with the field. Rotation that is asynchronous depends on the volume, drag and total magnetic moment of the particle, which is ideal for biosensing applications. These principles have been previously applied with ferromagnetic particles for viscosity sensing, pH sensing, and single bacterial cell detection. The principle of using asynchronous magnetic rotation for biodetection is illustrated in Figure 1, using paramagnetically labeled biotin and streptavidin particles to mimic an antibody-antigen system. The rate of rotation of the particle cluster increases with particle concentration over 2 orders of magnitude, demonstrating the potential for using this system as a biosensor.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 21, 2010
Pages: 7 - 10
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topicss: Chemical, Physical & Bio-Sensors, Diagnostics & Bioimaging
ISBN: 978-1-4398-3415-2