Targeted Cancer Therapy by Immunoconjugated Gold-Gold Sulfide Nanoparticles Using Protein-G as a Cofactor

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Gold-gold sulfide nanoparticle (GGS-NP) with controllable near infrared (nIR) absorption was synthesized from a simple reaction of chloroauric acid and sodium thiosulfate. Through PEGylated recombinant Protein-G (ProG), ProG-conjugated GGS-NPs for antibody capture by Fc domain provided a practical stratagem for early-stage cancer diagnosis and targeted therapy. In vitro studies, the as-fabricated ProG-conjugated GGS-NPs with bound anti erbB-2 (HER-2) IgGs successfully targeted at HER-2 overexpressing breast cancer cell, SK-BR-3, and killed them at an optimized low laser power. The concept of the targeted photothermal cancer therapy using ProG as a cofactor for immobilization of antibody is well proved and very promising as provides a repeatable platform method for antibody conjugation to gold nanoparticles.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Published: June 18, 2012
Pages: 238 - 241
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topicss: Biomaterials, Cancer Nanotechnology
ISBN: 978-1-4665-6276-9