Papers – Page 718 – TechConnect Briefs

Differential lethality of gold nanoparticles is associated with immunogenecity

Huang G.S., Chen Y-S, Yeh H-W, National Chiao Tung University, TW

To obtain antibodies specifically recognizing gold nanoparticles (GNPs) BALB/C mice were immunized by weekly intra-peritoneal injection of adjuvant-emulsified 5 nm, 8 nm, 12 nm, 17 nm, and 37 nm GNPs. Mice injected with 8 nm, [...]

Design of Magnetic Nanoparticles for Purification of His-tagged Recombinant Proteins

Mohapatra S., Pal D., Ghosh S.K., Pramanik P., Indian Institute of Technology, IN

Manipulation of proteins effectively for in-vitro study is an important step of research in the area of proteomics. The expression of recombinant proteins with histidine fusion tags(6xHis) is widely used to ease their purification from [...]

Creation of an Aptamer Nanobiosensor for Ultra-Sensitive Detection of Heart Failure

Darnaud M., Ricketts A., Graham D., University of Strathclyde, UK

This project focuses on discovering a new aptamer sequence that binds specifically and with high affinity to Brain Natriuretic Peptide (BNP), which is indicative of the early stages of heart failure. A library of random [...]

The Potential use of Fluorescent Nanoparticles from Hydrothermal Synthesis in Cell Imaging

Hobbs H., Briddon S., Lester E., The University of Nottingham, UK

We are using self fluorescing nanoparticles from a nozzle continuous hydrothermal synthesis reactor cell signalling. Most established methods for producing nanoparticles involve relatively noxious chemicals, have a complex and time-consuming sequence of stages, or may [...]

Fluorescent Nanoparticles Based on Chitosan

Bodnar M., Minko T., Hartmann J.F., Borbely J., University of Debrecen, HU

Chitosan is a basic linear polysaccharide containing β-[1→4]-linked 2-acetamido-2-deoxy-D-glucopyranose and 2-amino-2-deoxy-D-glucopyranose units. Because of the presence of reactive amino groups, chitosan can be modified easily to create nano- and micro particulate systems, films or porous [...]

A Fluorescence Spectroscopy Study on the Encapsulation of Hydrophobic Dyes in Core-Shell Nanoparticles

Chavez J.L., Wong J.L., Duran R.S., University of Florida, US

Novel core-shell nanoparticles were prepared as encapsulating agents for fluorescent organic dyes. These particles protect the dyes from polar solvents, allowing their use in aqueous environments, such as biological systems. The nanoparticles were synthesized using [...]

Single Base Mismatch Determination using DNA – Silver Nanoparticle Conjugates

Thompson D., Faulds K., Smith W.E., Graham D., University of Strathclyde, UK

Modification of oligonucleotides with gold nanoparticles was first reported ten years ago and opened up several new avenues of research as they can be used as a novel nanostructured building material but the conjugate can [...]

A pbpk model to elucidate processes governing distribution and excretion of polyacrylamide nanoparticles

Wenger Y., Jolliet O., Schneider R., Philbert M., University of Michigan, US

This paper presents a physiologically based pharmacokinetics model that has been calibrated using experimental measurements of biodistribution and excretion of polyacrylamide nanoparticles in rats. The model is capable of establishing the complete mass balance of [...]

Quantum Dots for Multimodal Bioimaging and Sensing Applications

Santra S., Yang H., Sabui S.K., Sharma P., Holloway P.H., Walter G.A., Moudgil B.M., Scott E., University of Central Florida, US

Fluorescent quantum dots (Qdots) have demonstrated their potential in diagnostic bioimaging applications in vitro. For in vivo bioimaging applications, however, the embodiment of additional properties such as paramagnetism onto the same fluorescent probes is highly [...]

Functionalized Quantum Dot Characterization and Applications in Neurosciences

Pathak S., Davidson M., Sharp C., Silva G., UCSD, US

Quantum dot nanocrystals are nanometer sized semiconductor particles, which can be used for labeling biological systems. A critical issue that has not been addressed is determining the number of antibodies bound to nanoparticles. We have [...]