Worsfold O., Nishiya T.
Fujirebio Inc., JP
Keywords: bionanosensor, integrin, lipid membrane, optical
An ultra-sensitive optical bionanosensor platform for integrin avb3 loaded human umbilical vein endothelial cells (HUVECs) was fabricated. The bionanosensor design involved the incorporation of an RGD (arginine-glycineaspartate) containing peptide conjugated BODIPY lipid soluble dye (donor) into a substrate supported bilayer lipid membrane (SBLM). The RGD peptide binds with the integrin avb3 via a multimerisation mechanism thus reducing the energy transfer from the donor dye moiety to a second higher excitation wavelength BODIPY lipid soluble dye (acceptor) moiety. The reduction in energy transfer is visualised by an increase in the fluorescence intensity of the donor and a decrease in the fluorescence intensity of the acceptor. The surface chemistry of the substrate has a significant effect on the fluidity of the lipid bilayer (measured by Fluorescence Recovery after Photobleaching (FRAP)) and on the subsequent biosensing response. Using this novel bionanosensor architecture a sensitivity of 25 HUVEC cells/ml was recorded.
Journal: TechConnect Briefs
Volume: 1, Technical Proceedings of the 2004 NSTI Nanotechnology Conference and Trade Show, Volume 1
Published: March 7, 2004
Pages: 217 - 220
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topics: Biomaterials, Chemical, Physical & Bio-Sensors
ISBN: 0-9728422-7-6