Kell A.J., Tan S., Charlebois I., Boissinot M., Simard B.
National Research Council, CA
Keywords: DNA, label-free detection, nanoparticle, polymeric transduction
We have developed a nanoparticle-based DNA biosensor that can detect unlabelled ssDNA with good sensitivity (picomolar concentrations). The biosensor makes use of the optical changes of a cationic polymer interacting with ssDNA versus that of hybridized DNA to provide a fluorescence signal only when a specific hybridization event occurs (i.e. only when perfectly complementary DNA is present)
Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 21, 2010
Pages: 1 - 3
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topics: Chemical, Physical & Bio-Sensors, Diagnostics & Bioimaging
ISBN: 978-1-4398-3415-2