Kwon S.H., Hong B.J., Park H.Y., Park J.W., Knoll W., Park H.Y., Park J.W.
Center for Integrated Molecular System., KR
Keywords: dendron, DNA hybridization, kinetics, self-assembly, Sol Gel, SPFS
Since we observed that dendron-assembled surface providing a proper spacing between immobilized DNAs showed high single nucleotide polymorphism discrimination efficiency for DNA microarrays(1), the nanoscale-controlled surface is examined with surface plasmon field-enhanced fluorescence spectroscopy (or SPFS) to elucidate the kinetics of the DNA hybridization. A silica film (d=33 nm) was coated onto a gold surface using the sol-gel technique to suppress fluorescence quenching, followed by self-assembly of a second-generation dendron and covalent immobilization of a catcher DNA strand at the dendron’s apex. The association rate for the DNA hybridization on the surface was faster than that reported for a streptavidin-modified surface. (2) In addition, we observed that the capability to capture DNA target strands was maintained even after extended storage at ambient conditions.
Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 20, 2007
Pages: 457 - 460
Industry sector: Sensors, MEMS, Electronics
Topics: Biomaterials, Chemical, Physical & Bio-Sensors
ISBN: 1-4200-6183-6