Maki G., Maki W., Mishra N., Fellegy M., Cameron E., Maki G., Maki W.
University of Idaho, US
Keywords: nano surface, nanowire, silicon oxide film, surface modification
Nano-scale Field Effect Transistor based devices (nano-FETs) have shown great promise in bio-molecular detection. The advantages of using electronic nano-devices verses using conventional bio-detection methods are: direct detection without labeling, ultra-sensitivity without amplification and simple device implementation without expensive equipment. However, immobilization of bio-molecules on nano-scaled devices is a challenge. We investigated methods for immobilizing oligonucleotide and peptide nucleic acid (PNA) on nanowire devices. Oligonucleotide and PNA were covalently linked on the nanowire surface which is covered by 1-2 nm native silicon oxide. Standard silanization chemistry combined with bi-functional linker approach was used in the immobilization process. Low concentration acetic acid solution was used to wash the silanized surface which reduced the thickness of the silane layer under 4 nm. This allows negatively charged target nucleic acids much closer to the nanowire, and increases detection sensitivity. Streptavidin-Alkaline phosphotase (SA-AP) conjugate was used as indicator to confirm immobilized oligonucleotide. PNA immobilization was confirmed by hybridization with a biotin labeled oligonucleotide with complimentary sequence, and detected by SA-AP conjugate.
Journal: TechConnect Briefs
Volume: 1, Nanotechnology 2010: Advanced Materials, CNTs, Particles, Films and Composites
Published: June 21, 2010
Pages: 681 - 684
Industry sector: Advanced Materials & Manufacturing
Topics: Advanced Materials for Engineering Applications, Coatings, Surfaces & Membranes
ISBN: 978-1-4398-3401-5