Lehmann U., Parashar V.K., Vandevyver C., Gijs M.A.M.
EPFL, CH
Keywords: bio-analysis, DNA, droplets, lab-on-a-chip, Magnetic Particles, microfluidics
We present a chip-based bio-analysis system using the two dimensional (2D) magnetic manipulation of microdroplets. The latter contain magnetic microparticles which serve as force mediators for the droplet actuation. In combination with a changeable field topology over the chip surface, we are able to perform all droplet manipulation steps, like transport, merging, mixing and splitting of droplets on our chip, as presented recently [1]. Based on such a droplet manipulation system, we implemented different bio-analysis protocols such as the on-chip colorimetric detection of biologically active markers and the on-chip purification of DNA. Both procedures show excellent results and demonstrate the potential use of the two-dimensional magnetic droplet manipulation system as a platform for miniaturized bio-analytical systems.
Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2006 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 7, 2006
Pages: 477 - 480
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topic: Micro & Bio Fluidics, Lab-on-Chip
ISBN: 0-9767985-7-3