Michaelis Menten – TechConnect Briefs

Enhanced Kinetics of Enzymatic Nanosensors

Breger J., Walper S., Ancona M., Stewart M., Susumu K., Medintz I., Naval Research Laboratory, US

Nanosensors employing quantum dots (QDs) and functional moieties such as enzymes are promising for diagnostics and chemical/biological threat activity. Their small size permits cell penetration and their inherent photochemical properties are well-suited for rapid, optical [...]

Determination of the bi-substrate kinetic coefficients for the beta-D-glucose- NAD-GDH enzymatic reaction

Berthier J., Cubizolles M., Laurent P., Pouteau P., Poher V., Cosnier M.L., CEA, FR

Colorimetric detection of glucose in sample liquids such as human plasma is made by using enzymatic reactions. In the multi reactional scheme, the first enzymatic reaction is determinant. Either glucose oxidase (GOX) or glucose dehydrogenase [...]

A Model for Enhanced Chemiluminescence Reactions in Microsystems

Berthier J., Joly P., Rivera F., Caillat P., CEA-LETI, FR

Detection of hybridized targets—like DNA strands—in microsystems for biotechnology is usually done by fluorescence. However, recent investigations have shown that chemiluminescence could be a more effective and sensitive tool for detection under the condition that [...]