diagnostics – TechConnect Briefs

New Technologies for the Rapid Identification of Drug-Resistant Bacteria

Sauer-Budge A.F., Exponent and Boston University, US

The extensive use of antibiotics to treat infections, without first specifically identifying the underlying cause, has resulted in a strong natural selection for antibiotic resistant organisms. Conservative estimates from the CDC indicate that more than [...]

Customized Nanoparticle Probes for Biomedical Imaging, Diagnostics and Therapeutics Applications

Nallathamby P., University of Notre Dame, US

The modular synthetic approach that was developed to design a spectral library of core-shell nanoparticle contrast agents, which will have broad applications in biomedical imaging due to the potential for multi-modal imaging (e.g., fluorescence, MRI, [...]

Quantitative Electrolateral Flow Immunosensor (ELLI): Dengue Triage and Stroke Prognosis Case Studies

Sinawang P.D., Fajs L., Marks R.S., Ben-Gurion University of the Negev, IL

The development of a rapid, affordable, and sensitive diagnostic kit for point-of-care is important in most healthcare settings; however, only a few exhibit the needed quantitative capability. Our technology, ELLI, is a patented diagnostic device [...]

Amplification-free liquid biopsy by fluorescence approach

Uhd J., Taskova M., Okholm A., Astakhova K., University of Southern Denmark, DK

Diagnostics of cancer associated ctDNA, BRAF V600E, KRAS G12D and KRAS G13D respectively. By the use of modern fluorescence microscopy and fluorescently labeled mutation specific LNA/DNA probes. Which resulted in a mutation specific detection.

Microfluidics for multiplexing of core needle biopsies

Mojica W.D., Oh K.W., Lee H., Xue X., Furlani E.P., University at Buffalo, US

Image guided biopsies for the procurement of diagnostic tissue has proven to be safe, cost effective and useful. However, the amount of tissue obtained is often diminutive and may be exhausted for conventional diagnostic purposes. [...]

Microfluidics for multiplexing of core needle biopsies

Mojica W.D., Oh K.W., Lee H., Xue X., Furlani E.P., University at Buffalo, US

Image guided biopsies for the procurement of diagnostic tissue has proven to be safe, cost effective and useful. However, the amount of tissue obtained is often diminutive and may be exhausted for conventional diagnostic purposes. [...]

Dielectrophoretic Isolation and Detection of Cancer Related DNA and RNA Nanoparticulate Biomarkers from Blood and Plasma

Heller M.J., Marciniak J., Skowronski E., Sonnenberg A., University of California San Diego, US

The analysis of cell free circulating (cfc) DNA and RNA nanoparticulate biomarkers continues to become more widely used for cancer diagnostics and management. Generally, cfc-DNA for “liquid biopsies” is isolated form plasma using relatively long [...]

Synthetic LNA/DNA Nano-scaffolds for Highly Efficient Diagnostics of Nucleic Acids and Autoimmune Antibodies

Astakhova I.K., University of Southern Denmark, DK

Herein we describe fluorescent oligonucleotides prepared by click chemistry between novel alkyne-modified locked nucleic acid (LNA) strands and a series of fluorescent azides for highly efficient homogeneous (all-in-solution) detection of nucleic acids and autoimmune antibodies. [...]

Isolation and Detection of Cancer Related DNA Nanoparticulate Biomarkers Directly from CLL Patient Blood “Rapid Sample to Sequence Diagnostics”

Heller M.J., Marciniak J., Sonnenberg A., University of California San Diego, US

The analysis of cell free circulating (cfc) DNA biomarkers continues to become more widely used for cancer diagnostics and management. Cfc-DNA ranges from nucleosomes and high molecular weight DNA nanoparticulates to 100-200bp fragments. We have [...]

Magnetization of hydrogel particles: a novel urine tuberculosis antigen test

Paris L., Cox A., Petricoin E., Liotta L., Luchini A., George Mason University, US

This study aims at developing a nanotechnology-based screening test that would reliably detect early stage tuberculosis (TB) infection and discriminate active from latent infections. The use of urine in TB testing has been hampered by [...]