cell separation – TechConnect Briefs

Size-Dependent Separation of Human Mesenchymal Stem Cells by Applying Microfluidic Chip Filtration

Yu S.-Y., Yoo J-H, Chun M.-S., Chang M.-S., Korea Institute of Science and Technology (KIST), KR

It is important to separate human bone marrow-derived mesenchymal stem cells (hMSCs) with high multipotential to differentiation and to increase efficacy of cell therapy. We investigated to develop and validate a novel method for collection [...]

A tag-less method for the direct isolation of HUVEC (human umbilical vein endothelial cells) by gravitational field-flow fractionation as a starting point for gene expression analysis

Lattuada D., Roda B., Pignatari C., Magni R., Cattaneo A., Bolis G., Reschiglian P., University of Bologna, IT

HUVECs (human umbilical vein endothelial cells) are a model for prediction of endothelial dysfunction in various diseases. HUVECs are enzymatically removed from the umbilical vein by collagenase. This method allows for a good sample yield, [...]

A Model for Blood Cell Transport and Separation in a Magnetophoretic Microsystem

Furlani E.P., University at Buffalo, US

We present a model for predicting the transport and separation of red and white blood cells in plasma in a magnetophoretic microsystem. The microsystem is passive and consists of an array of integrated soft-magnetic elements [...]

Simulation and Optimization of a Flow-Through Micro PCR Chip

Kang J., Chen C.C., Smolinski J., Thomas R.A., Tucker J.D., Auner G.W., Industrial Technology Research Institute, TW & Wayne State University, US

We present a microfluidic particle concentrator and separator employing a large flow unit array. Each flow unit includes a nozzle, a sharp turn, a diffuser, and a flow dividing segment. After acceleration in the nozzle, [...]

Cell Separation and Manipulation by magnetic field in the presence of functionalised magnetic nanowires

Prina-Mello A., Diao Z., Coey J.M.D., CRANN Research Laboratory, IE

In this paper we investigate the possibility of using magnetic nanowires in cell separation and manipulation. Ni, Co/Cr and Co/Fe nanowires made by electrochemical deposition in anodic aluminum oxide (AAO) templates. The combination between nanowires [...]