Mishra N.N. – TechConnect Briefs

Fluorescence Based Specific PNA-DNA Binding Characterization on nano-FET DNA Device Surface

Rastogi S.K., Mishra N.N., Finaloski B., Cameron E., Maki W., Postdoc Researcher, US

A novel sequence of peptide nucleic acid (PNA; beta-Ala-(T10)-beta-Ala-NH2) containing ten thymine monomers and beta-alanine amino acid at both (C and N) terminal of main chain was synthesized manually using solid phase tBoc peptide chemistry. [...]

Water Soluble Peroxyoamid Reagent: Evalulation for Fluorescent Silica Nano-Particles Chemilumineacence

Rastogi S.K., Fianaloski B., Mishra N.N., Cameron E., Bitterwolf T.E., Branen A.L., Maki W.C., University of Idaho, US

Chemiluminescence methods, both enzymatic and non-enzymatic, are widely used as an analytical tool in various bio-molecular detection applications. Advantages of using chemiluminscence detection are its practical simplicity, relatively low back ground interference, high sensitivity compared [...]

Polysilicon Nano-wires Based Nano-device on Silicon Chip: Fabrication and its Application

Mishra N.N., Winterrowd P., Nelson R., Maki G., Maki W.C., Rastogi S.K., Cameron E., Filanoski B., Maki G., Maki W.C., University of Idaho, US

5x5 arrays of six nano-transistor devices were fabricated on p-type silicon/silicon oxide (200nm) wafers with resistivity 1-100 -cm. 50nm of doped polysilicon were deposited and annealed to make nanowires. All nanowires were made with direct [...]

Ultra-sensitive Electrochemical Detection of E. coli Using Nano-porous Alumina Membrane

Mishra N.N., Maki G., Maki W.C., Filanoski B., Fellegy M., Cameron E., Rastogi S.K., Maki G., Maki W.C., University of Idaho, US

A combinational method has been developed for ultra-sensitive detection of E. coli. The method combined biological, nano-fabrication and electrochemical techniques to achieve highly specific and ultra-sensitive detection of bacterial pathogen. E. coli K12 was used [...]

Polysilicon Nano-wires Based Nano-device on Silicon Chip: Fabrication and its Application

Mishra N.N., Winterrowd P., Nelson R., Maki G., Maki W.C., Rastogi S.K., Cameron E., Filanoski B., Maki G., Maki W.C., University of Idaho, US

5x5 arrays of six nano-transistor devices were fabricated on p-type silicon/silicon oxide (200nm) wafers with resistivity 1-100 -cm. 50nm of doped polysilicon were deposited and annealed to make nanowires. All nanowires were made with direct [...]

Functionalized Mesoporous Silica Nano-particle for Optical Bio-detection

Maki G., Maki W.C., Mishra N.N., Filanoski B., Rastogi S.K., Cameron E., Maki G., Maki W.C., University of Idaho, US

Mesoporous silica nano-particles were immobilized with luciferase and antibodies for immune-bioluminescence detection of Staphylocuccus aureus enterotoxin B (SEB) and C (SEC). Mesoporous silica nano-particles were used as matrix to immobilize antibodies specific for enterotoxins and [...]

Bio-Impedance Sensing Device (BISD) for Detection of Human CD4+ Cells

Mishra N.N., Retterrer S., Zieziulewicz T.J., Isaacson M., Szarowski D., Mousseau D.E., Lawrence D.A., Turner J.N., New York City Department of Health, US

The aim of this work is to develop a small on-chip bio-impedance sensing device (BISD) to rapidly detect and quantify cells with a specific phenotype in a heterogenous population of cells (e.g., human CD4+ cells [...]