Feng J.J. – TechConnect Briefs

A Continuous Particle/Cell Sorter Using Dielectrophoresis

Wang G.R., Feng J.J., Vasantgadkar S., Pant K., Prabhakarpandian B., Krishnamoorthy S., Sundaram S., CFD Research Corporation, US

We present a novel design of a continuous microfluidic sorter for separation of cells or particles by the intelligent use of conventional negative dielectrophoresis with separation efficiency of greater than 95%. A high fidelity modeling [...]

Simulation of Electrokinetic Flow and Analyte Transport in Nano Channels

Feng J.J., Krishnamoorthy S., Wang G.W., Sundaram S., CFD Research Corporation, US

This paper demonstrates the electrokinetic flow and transport of charged and neutral analytes in nanochannels by solving full system of governing Poisson-Nernst-Planck equations without any assumptions. The issues related to the spatial and time scales [...]

A Multi-scale, Integrative Model of Cellular Electrophysiological Response Incorporating Intra/Extra-cellular Transport & Dynamics

Feng J.J., Krishnamoorthy S., Sundaram S., CFD Research Corporation, US

Spurred by the growing confluence of nano and bio technologies, several novel micro/nano platforms (see for example, whole cell patch clamp devices [1]) are emerging for the high-throughput and/or high-fidelity study of the electrophysiological response [...]

Numerical and Analytical Studies of Electrothermally Induced Flow And Its Application For Mixing and Cleaning in Microfluidic Systems

Feng J.J., Krishnamoorthy S., Sandaram S., CFD Research Corporation, US

This paper presents both analytic and simulation results of electrothermal flow in microfluidic systems. It also considers the application of this phenomenon to microscale mixing and cleaning. The basic operation requires application of an alternating [...]

Development and Characterization of a 2-D microchip-based Protein separation system

Feng J.J., Olazábal V, Olivares J.A., Sundaram S., Krishnamoorthy S., Iyer S., Los Alamos National Laboratory, US

Traditional methods in proteomics have relied on gel-based separation of proteins followed by directed cleavage of these proteins and mass spectrometry to measure the products. The emphasis on reliable, high-throughput analyses has led to the [...]

Numerical Simulation of Field Amplified Sample Stacking in Microfluidic System

Feng J.J., Krishnamoorthy S., Sundaram S., Bhardwaj R., Santiago J.G., CFD Research Corporation, US

In this paper we developed a numerical simulation method that solves the transport phenomenon associated with sample stacking in microchip-based separation system. The model is based on thin double layer approximation where the electrostatic force [...]

Numerical and Analytical Studies of AC Electric Field in Dielectrophoretic Electrode Arrays

Feng J.J., Krishnamoorthy S., Chen Z.J., Makhijani V.B., CFD Research Corporation, US

Manipulation of micro-sized particles and biological cells using dielectrophoresis (DEP) is an emerging technique in MEMS and nano technology[3]. This paper presents an exact solution of dielectrophoretic motion of a polarized particle in the vicinity [...]

Analysis of Physico-Chemical Processes in an Amperometric Oxygen Biosensor

Krishnamoorthy S., Feng J.J., Makhijani V.B., Gao C., Choi J.W., Ahn C., CFD Research Corporation, US

Amperometric techniques are commonly used in lab-on-a-chip systems to sense various blood gas analytes such as H+, CO2, O2, etc. In the present study, a microchip-based amperometric biosensor, being developed as a part of plastic-based [...]

Numerical and Analytical Studies of AC Electric Field in Dielectrophoretic Electrode Arrays

Feng J.J., Krishnamoorthy S., Chen Z.J., Makhijani V.B., CFD Research Corporation, US

Manipulation of micro-sized particles and biological cells using dielectrophoresis (DEP) is an emerging technique in MEMS and nano technology[3]. This paper presents an exact solution of dielectrophoretic motion of a polarized particle in the vicinity [...]

Analysis of Sample Transport in Capillary Electrophoresis Microchip Using Full-Scale Numerical Analysis

Krishnamoorthy S., Feng J.J., Makhijani V.B., CFD Research Corporation, US

An implicit finite-volume numerical scheme has been developed to solve the three-dimensional transport equations along with fully-coupled ionization equilibria in acid/base systems. The scheme solves conservation equations for mass and momentum while satisfying the electroneutrality [...]