Chung C. – TechConnect Briefs

Genetic Algorithm for the Design of Microchip Flow Cytometers

Bang H., Lee W.G., Yun H., Min J.K., Chung C., Chang J.K., Han D-C, Seoul National University, KR

Robot designers and artificial-intelligence researchers have long been inspired by living things. Now, we are trying to mimic some of the most fundamental features of life while designing micro devices. In this paper we proved [...]

In Situ Investigation of Uptake Phenomena of Biological Molecules and Silica Nanoparticles into Mammalian Cells in Microstructures

Lee W.G., Bang H., Yun H., Kim J.A., Cho K., Shin Y.S., Chung C., Chung N.C., Chung C., Chung N.C., Chang J.K., Han D-C, Seoul National University, KR

Electroporation is a powerful gene transfection method to deliver foreign biological molecules such as DNA and protein into living cells with electric pulsations. However, conventional cuvette-type electroporation techniques generally suffer from poor transfection efficiency and [...]

Active Sealing for Soft Poymer Microchips

Bang H., Lee J., Lee W.G., Park J., Yun H., Lee J., Lee W.G., Chung C., Chung S., Cho K., Chung C., Chung S., Han D-C, Chang J.K., Seoul National University, KR

This paper presents a universal sealing method for soft polymer (elastomer) microchips. A robust and reversible sealing method which allows various materials to be bonded and sealed tightly with each other even in aqueous solutions, [...]

Conjugation of DNA to Streptavidin-coated Quantum Dots for the Real-time Imaging of Gene Transfer into Live Cells

Kim J.K., Lim S.H., Lee Y., Shin Y.S., Chung C., Yoo J.Y., Chang J.K., Digital Bio Technology, Co, KR

We have developed the method for the conjugation of biotinylated DNA to streptavidin-coated quantum dots (QDs). QD-DNA conjugates and a highly sensitive fluorescence imaging technique are adopted to visualize gene transport across the membrane of [...]

Highly Efficient Electro-permeabilization of Mammalian Cells Using Micro-electroporation Chip

Shin Y.S., Kim J.K., Lim S.H., Lee Y., Lee Y.K., Cho K., Lee Y., Lee Y.K., Chung C., Chung S., Chung C., Chung S., Han D-C, Chang J.K., Seoul National University, KR

Electroporation, is widely used method that can be directly applied to the field of gene therapy. However, very little is known about the basic mechanisms of DNA transfer and cell response to the electric pulse. [...]

Microfabricated Fluorescence-Activated Cell Sorter with Hydrodynamic Flow Manipulation

Bang H., Chung C., Chung S., Kim J.K., Kim S., Kim J.K., Kim S., Chung C., Chung S., Han D-C, Chang J.K., School of Mechanical & Aerospace Eng., Seoul National University, KR

Presented is a novel flow manipulation and particle detection method for the microfabricated fluorescence-activated cell sorter (FACS). With hydrodynamic flow manipulation which includes passive flow channeling and hydrodynamic actuation with nozzle flow, we have developed [...]