Kim J.K., Lim S.H., Lee Y., Shin Y.S., Chung C., Yoo J.Y., Chang J.K.
Digital Bio Technology, Co, KR
Keywords: conjugation, gene transfer, live cell imaging, quantum dot, semiconductor nanocrystal
We have developed the method for the conjugation of biotinylated DNA to streptavidin-coated quantum dots (QDs). QD-DNA conjugates and a highly sensitive fluorescence imaging technique are adopted to visualize gene transport across the membrane of the live cell in real time. Endocytotic cellular uptake of oligonucleotide is monitored by a real-time confocal imaging system. Long-term kinetic study enables us to reveal the unknown mechanisms and rate-limiting steps of extracellular and intracellular transport of DNA. Gel electrophoresis is used to verify the effect of incubation time and the molar ratio of QDs to DNA on the conjugation efficiency. It is possible to fractionate the QD-DNA conjugates according to the DNA concentration and obtain the purified conjugates by a gel extraction technique. QD-DNA conjugates have a potential to be nanoscale building blocks by self-assembly process as well as a versatile tool for fluorescence imaging and monitoring of biological systems.
Journal: TechConnect Briefs
Volume: 3, Technical Proceedings of the 2004 NSTI Nanotechnology Conference and Trade Show, Volume 3
Published: March 7, 2004
Pages: 379 - 382
Industry sector: Advanced Materials & Manufacturing
Topic: Nanoparticle Synthesis & Applications
ISBN: 0-9728422-9-2