Papers – Page 52 – TechConnect Briefs

Quantitative Detection by Single Ion Channel Event Statistics: an Algorithmic Approach

Lodesani F., Bennati M., Fiegna C., Tartagni M., ARCES, IT

The capability of having ligand-gated ion channels embedded in planar biomimetic membranes, together with a proper electronic system for elaborating the signal will give the possibility of creating high-performances sensors, able to detect low concentrations [...]

Identifying Nanoscale Molecular Binding Events With SPM/AFM

Johnson W.T., Agilent Technologies, US

PicoTRECTM is a chemical/biological detection accessory for the Agilent SPM/AFM (scanning probe microscopy/atomic force microscopy) that combines molecular scale imaging with nanoscale, molecular recognition mapping. It is being used to map interactions between antibodies/antigens and [...]

Single Molecule Interaction Forces and Recognition Imaging

Kienberger F., Chtcheglova L.A., Ebner A., Puntheeraunrak T., Gruber H.J., Hinterdorfer P., University of Linz, AT

In recent years, considerable attention has focused on biological applications of the atomic force microscope (AFM), in particular on high-resolution imaging of individual biological molecules and on the measurement of molecular forces under near-physiological conditions. [...]

Silver Particle and Silver Thiolate-Based Molecular Sensing/Recognition Units Operating via Surface-Enhanced Raman Scattering

Kim K., Kim N.H., Park H.K., Kim K., Kim N.H., Lee H.S., Lee Y.M., Lee H.S., Lee Y.M., Seoul National University, KR

Noble metallic nanostructures exhibit a phenomenon known as surface-enhanced Raman scattering (SERS) in which the scattering cross sections are dramatically enhanced for molecules adsorbed thereon. We demonstrate that commercially available Ag(μAg) powders can be used [...]

Development of SERS Spectroscopy for Routine and Rapid Identification of Escherichia Coli and Listeria Monocytogenes on Ag Colloidal Nanoparticles

Chen Y-R, Liu Y., Nou X., Chao K., USDA/ARS Beltsville Agricultural Research Center, US

Rapid, accurate, and preferably routine methods for the identification of foodborne bacteria are increasingly important, because of bio- /agro-terrorism threats, public health concerns, and economic loss. Conventional detection of bacteria is time-consuming, requiring 5-7 days, [...]

Parallel two channel near and far field fluorescence microscopy

Verdes D., Rabe M., Seeger S., University of Zurich, CH

We report a new two channel fluorescence microscopy technique for surface-generated fluorescence. The realized fluorescence microscope allows high resolution imaging of aqueous samples. The core element of the instrument is a parabolic mirror objective that [...]

Investigation of the Conductance of Silicon Nanowire Biosensors Using the 2D Drift-diffusion Model

Damodaran S., Vadivelmurugan S., Do Q.T., Heitzinger C., Liu Y., Klimeck G., Purdue University, US

DNA-FETs are DNA sensors consisting of a semiconductor transducer and a functionalized surface. Their device structure is similar to a MOSFET, but the gate has been replaced by the functionalized surface. Since DNA-FETs allow direct [...]

Utilizing Silver and Gold Nanoparticles for Investigation of Bacterial Cell Wall Biochemical Structure

Culha M., Kahraman M., Yazici M.M., Sahin F., Yeditepe University, TR

Understanding fundamental principles of living systems in their natural environment is always a challenge for scientists. The desire to design and construct novel diagnostic tools makes this understanding a significant prerequisite. Due to its “fingerprinting” [...]

Encapsulation of Streptavidin labeled with Alexa Fluor 750 into Erythrocytes

Dweik M., Grant S.A., University of Missouri-Columbia, US

Erythrocytes, Red Blood Cells (RBCs), have been utilized as biocompatible bioreactors and drug delivery vehicles. RBCs are also used as biological carriers for drugs, enzymes, and peptides. Hypo-osmotic dialysis technique is the most commonly employed [...]

Prevention and Risk Reduction in Heart Health

Kaminska B., Tavakolian K., Ngui B., Chuo Y., Carmichael B., Simon Fraser University, CA

Identifying early onset of cardiac disease is not easy and it is very expensive. For this reason the first symptom of cardiac problems is often catastrophic. It is the goal to produce a low cost [...]