Papers – Page 32 – TechConnect Briefs

A Lab-on-a-Chip using Magnetic Droplets

Lehmann U., Parashar V.K., Vandevyver C., Gijs M.A.M., EPFL, CH

We present a chip-based bio-analysis system using the two dimensional (2D) magnetic manipulation of microdroplets. The latter contain magnetic microparticles which serve as force mediators for the droplet actuation. In combination with a changeable field [...]

Three-Dimensional Reconstruction of the Florid Plaque in variant Creutzfeldt-Jakob Diseases

Zubert M., Napieralska M., Napieralski A., Sikorska B., Liberski P., Grams R., Technical University of Lodz, PL

The Alzheimer, BSE , CJD and vCJD diseases belongs to a group of neurodegenerative diseases characterised by an accumulation of extraneuronal filamentous material consisting of β-sheet proteins of various biochemical properties. Clinically, these diseases manifest [...]

Neuroimaging Distribution of Water Molecules in Brain

Vaganova E., Yitzchaik S., Ovadia H., Borst J.W., Visser A., Sigalov M., Khodorkovsky V., The Hebrew University, IL

We were able to elucidate and build the map of the water molecules distribution in a brain tissue in hippocampus/lateral vetricle area by means of the injected chromophore bis-N-carbazolyl-distyrylbenzene (BND) and FLIM technique. The BND [...]

Quantum Dot Probes for Tracking Nerve Growth Factor Ligand-Receptor Dynamics in Live Neurons

Sundara Rajan S., Vu T.Q., Oregon Health and Science University, US

The mechanisms by which cell-surface receptors interact with their specific ligands to initiate signaling cascades is a dynamic process which involves ligand-receptor binding, transport, and clearance across and within the cell plasma membrane. Nerve growth [...]

Labeling of Cells with Silver and Gold Host-Guest Nanocomposites

Balogh L.P., Lesniak W., Sinani V.A., Bielinska A.U., Sun K., Nair B.M., Waite E., Kariapper M.S.T., Khan M.K., Roswell Park Cancer Institute, US

Nanocomposites combine the properties of nano-sized inorganic guest atoms/molecules with their nanoscopic size polymer host. We have synthesized water-soluble, biocompatible, fluorescent, and stable nanocomposites with regulated surface charge and controlled size for cell labeling studies. [...]

Mounting proteins on metal nanoparticles: Statistical analysis of AFM images

Gurevich L., Fojan P., Saxena R.K., Petersen S.B., Aalborg University, DK

In this work we assess the use of atomic force microscopy (AFM) study combined with activity assays, DLS and fluorescent measurements for complex characterization of nanoparticle-protein conjugates. Image processing techniques were used to locate and [...]

Synthesis and differential uptake of drug-decorated fluorescent nanoparticles by live cancer cells

Rozhkov R.V., Knudsen G.M., Sturgis J., Ribbe A.E., Robinson J.P., Davisson V.J., Purdue University, US

Design and applications of “smart” multivalent fluorescent probes based on semiconductor nanocrystals and anticancer drug molecules will be presented. Various polymer coating strategies have been employed to insure stability and define composition of nanoparticles. Next, [...]

Visualization of quantum dot-electron donor conjugate uptake and processing by living cells

Clarke S.J., Hollmann C.A., Nadeau J.L., McGill University, CA

The interactions of semiconductor quantum dots (QDs) with living cells remain poorly understood. It is known that certain sizes (colors) of QDs penetrate more readily into subcellular compartments, such as the nucleus, or into bacteria. [...]

Preparation of Aggregation Stable Block Copolymer Nanoparticles for Simultaneous Drug Delivery and Imaging

Gindy M.E., Prud’homme R.K., Princeton University, US

We have developed a nanoparticle formation process based on rapid micromixing to produce high supersaturations, which we term Flash NanoPreciptiation.4, 5 This work introduces a novel approach that expands the applications of the Flash NanoPrecipitation [...]

Rapid Isolation of AMI Markers using Nanomagnetic Particles

Haik Y., Sista R., Qablan A., Chen C-J., Palanki S., United Arab Emirates university, AE

Due to the increasing number of AMI patients accompanied with a difficulty in diagnosing the anomaly, a major push to develop innovative approaches to tackle the recognition of myocardial infarctions has been noticed. Most of [...]