Biomaterials – Page 125 – TechConnect Briefs

Immunosensing Using Fabricated protein Microarrays

Howell S.W., Inerowicz H.D., Guirl L., Regnier F., Reifenberger R., Purdue University, US

Patterned microarrays of antibodies to specific bacteria were used to perform a series of bacterial immunoassays of E. Coli 0157:H7 and Renibacterium Salmoninarum (RS). Microarrays were fabricated using microcontact printing (MCP) and characterized using scanning [...]

Microfluidic System For High-Throughput Proteomics

Le Gac S., Arscott S., Druon C., Tabourier P., Rolando C., USTL, FR

The comparative study of a cell type whole proteome in normal, pathological conditions or under the administration of drugs, which is called differential proteomics, appears as a keytool to understand diseases. To reach this goal, [...]

Microfluidic Technology Applied to Protein Sizing and Quantitation

Barthmaier P., Agilent Technologies, US

Although protein analysis technologies are developing fast, the current standard method is still denaturing gel electrophoresis (SDS-PAGE). Many researchers rely on this traditional method that has not substantially changed in the past 30 years. However, [...]

Particle Movement in Microelectrode Structures in AC Electric Fields, Under the Influence of Dielectrophoresis and Electrohydrodynamics

Green N.G., University of Glasgow, UK

The use of AC electric fields for the manipulation, characterisation and separation of particles in suspension (dielectrophoresis: DEP) has become an area of considerable interest, both in basic research of the underlying physical mechanisms [1] [...]

Bio Arrays and Proteomics

Glucose Sensor Based On Enzyme Entrapment In Polyacrylamide Microgels

Lopez-Cabarcos E., Retama B.R., Mecerreyes D., Lopez-Ruiz B., Universidad Complutense, ES

Glucose oxidase (GOx), was encapsulated in polyacrylamide microgels with the aim to preserve the enzymatic activity and to fabricate a biosensor with these microgel particles (1-3). The biosensor presents great stability, but when it is [...]

PCR Micro-Voume Device for Detection of Nucleic Acids

Auroux P-A, Day P., Niggli F., Manz A., Kinderspital, CH

The polymerase chain reaction (PCR) is an enzyme-mediated nucleic acid amplifer process that is employed extensively throughout the medical and biological sciences. The technique has risen to acclaim largely through its exquisite sensitivity, but an [...]

Molecular Recognition on Demand

Sherrill C., Marshall D., Richmond C., Scherrer C., Prudent J.R., EraGen Bioscience, Inc., US

DNA is the only chemistry that allows for ?molecular recognition on demand?. That is, unlike any other molecular recognition chemistry, DNA allows for the simple design and rapid synthesis of molecule sets that will recognize [...]

Nanoscale Bio-Molecular Control Using EC-OWLS

Bearinger J.P., Vörös J., Spencer N.D., Hubbell J.A., Textor M., ETH Zürich/ Lawrence Livermore National Laboratory, US

A new technique has been developed that combines evanescent-field optical sensing with electrochemical control of surface adsorption processes. This technique, termed Electrochemical Optical Waveguide Lightmode Spectroscopy (EC-OWLS), proved efficient in monitoring molecular surface adsorption and [...]

Topic: Biomaterials