Shen F.Y., Chen J.H., Chen N.H., Lam K.Y., Chen J.H., Chen N.H., Huang F.S.
National Tsing Hua University, TW
Keywords: Aminosilane, Anti-Mouse protein, contact imprinting, HSQ
The purpose of this paper is to develop nano-contact imprint technology with simple process by using soft HSQ (hydrogen silsesquioxane) mold transfer aminosilane ink on HSQ and glass substrates with binding Glutraic dialdehyde (GA) and Affinipure Goat Anti-Mouse igG(H+L) protein sequentially for bio application. HSQ molds were fabricated by low-dose e-beam lithography with various wires width, 80nm~200nm, the ratio of line width and space is 1:10 and 1:1, Aminosilane was used as ink, the nano-features on the HSQ stamps were successfully transferred onto the substrates with strong adhesion under specific conditions in the range of 350~450W O2 plasma treatment for 30 s and 10~35 psi imprinting pressure for 30s. We used AFM to measure the nano line before and after soaking with GA and Anti-Mouse igG(H+L) protein of transfer aminosilane pattern and to observe the protein was absorbed with aminosilane successfully. The layer thickness of GA with protein is about 5 nm. As we know, the size of the Anti-Mouse protein is 5nm*5nm*30nm. This indicates an approximate monolayer of absorbed igG(H+L) protein on the GA bound to patterned aminosilane nano line. Bio application on sensors and facilitate will be investigated further.
Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2008: Life Sciences, Medicine & Bio Materials – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: June 1, 2008
Pages: 270 - 273
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topics: Biomaterials, Materials Characterization & Imaging
ISBN: 978-1-4200-8504-4