The Interaction of Protein-Modified Nanodiamond with Bacterial Cells

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The possibility of using nanodiamond as bioactive nano-probes for bio-object labeling and treatment is demonstrated. The conjugation of Nanodiamond crystals and bio-molecules are comparatively new but promising subject of interest for bio-applications, due to their availability in various nano-size, chemical stability, and biocompatibility. The developed surface could have surface functional groups such as carbonyl- C=O, and hydroxyl-type C–O–H bonding as well as hydrocarbon fragments and the possibility of modifying the surface with biologically active molecules. The surface modification is key step in development of nanodiamond bio-probing and bio-sensing that has specific or non-specific interaction with bio-object. Here, we study the nanometer-sized diamond functionalization by creating functional groups on the surface and to optimize this process. The surface functionalization then followed by conjugating protein lysozyme via physical adsorption. Nanodiamonds surface properties and nanodiamond-protein interaction are analyzed using FTIR and UV/VIS spectroscopy. The interaction of lysozyme-nanodiamond complexes with E. Coli has been observed. These complexes label the bacteria and display high anibacterial activity. The results demonstrated the developed lysozyme-nanodiamond complexes preserved functionality of protein, while in the same time nanodiamonds served as probe using spectroscopic methods.

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Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2006 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 7, 2006
Pages: 440 - 443
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topic: Diagnostics & Bioimaging
ISBN: 0-9767985-7-3