Simultaneous Measurement of Nitric Oxide and Oxygen Dynamics during Myocardial Ischemia Reperfusion of Rat

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Myocardial function is dependent on a constant supply of oxygen from coronary circulation. Therefore the accurate measurement of oxygen concentration in postischemic myocardium is crucial to the assessment of myocardial viability and function. Nitric oxide (NO) plays important roles in the cardiovascular system by mediating various physiological and pathophysiological processes. However, the role of endogenous NO in ischemia-reperfusion is still controversial. Therefore, we utilized a sol-gel derived electrochemical NO and oxygen microsensor to monitor changes in NO and oxygen during myocardial ischemia-reperfusion. NO and oxygen sensors were built using a xerogel-modified platinum microsensor and coiled Ag/AgCl reference electrode. Hearts were placed on a Langendorff apparatus and treated by 30 min of ischemia and 60 min of reperfusion. In the ischemic myocardium, NO showed a time-course dependent change during the 30 min ischemic episode. And the restoration concentration of NO and oxygen was decreased below the pre-ischemic level after myocardial ischemia and early reperfusion. It seemed that endogenous NO formation was observed during ischemic episodes, which is a reaction against ischemia. And the oxygen recovery after ischemic episodes can be assessed by the extent of reoxygenation and the severity of ischemia.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2012: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Published: June 18, 2012
Pages: 60 - 63
Industry sector: Medical & Biotech
Topicss: Diagnostics & Bioimaging, Sensors - Chemical, Physical & Bio
ISBN: 978-1-4665-6276-9