Pant K., Romanowicz B.F., Sundaram S.
CFD Research Corporation, US
Keywords: assay, cell, design, microsphere, resolved particle simulations
Microsphere and cell-based assays are emerging as the platform of choice for rapid quantitation of biomolecular interactions in bioagent detection, clinical diagnosis, drug therapy, etc. [1,2] Traditional assay designs have been driven by expensive trial-and-error experimentation. The availability of high-fidelity simulation tools for these complex microsystems can help accelerate the design cycle significantly. However, current models rely on a point particle approximation and are incapable of describing large particle dynamics or biochemical coupling adequately. In a novel development, we have successfully developed computational models for finite-sized cells/bead assay design within a multiphysics biomicrofluidic simulation software suite, CFD-ACE+. The developed models fully integrate large bead dynamics, convective-diffusive analyte transport and biomolecular binding on bead surface. In this paper, we present the modeling framework, along with several validation and demonstration case studies.
Journal: TechConnect Briefs
Volume: 1, Technical Proceedings of the 2004 NSTI Nanotechnology Conference and Trade Show, Volume 1
Published: March 7, 2004
Pages: 184 - 187
Industry sector: Medical & Biotech
Topics: Biomaterials, Informatics, Modeling & Simulation
ISBN: 0-9728422-7-6