Papers – Page 868 – TechConnect Briefs

A Demonstration of High-throughput Immunoassay and Small Molecule Binding on Protein Microarrays with SPR Microscopy

Kim G., Jiang L., Rathod P.K., Campbell C.T., Nishimoto A., Casasanta V., University of Washington, US

The use of Surface Plasmon Resonance (SPR) for the detection of biomolecular binding events at the nanometer scale is of great utility when expanded into microarray density formats. Protein microarray analysis is particularly well suited [...]

Apoptosis of the Human Neutrophils under a Lipopolisaccharide (LPS) of Proteus Mirabilis Condition Observed in Real Time by Atomic Force Microscopy (AFM)

Pleskova S.N., Guschina Yu., Zvonkova M.B., Medical State Academy Nizhniy Novgorod, RU

The goal of present work was to receive the images of apoptosis process in dynamics after addition of Proteus mirabilis 210 LPS and to measure the mechanical properties change of neutrophils’ membranes. We observed the [...]

New Ferrocene-Functionalized Cationic Polythiophene For Label-Free Electrochemical DNA Detection

Harding-Lepage P., Le Floch F., Leclerc M., Ho H.-A., Harding-Lepage P., Leclerc M., Université Laval, CA

A specific polycationic polythiophene was discovered in order to identify dsDNA from ssDNA. The main properties of this new polymer are its conformational possibilities associated with photophysics modifications. This conjugated polymer is still subject to [...]

Cationic Polythiophene for Label-Free, Colorimetric and Fluorometric Detection of DNA

Ho H.-A., Leclerc M., Université Laval, CA

In order to respond to new demands and needs in modern medical diagnostics and biomedical research applications, a lot of new DNA detection approaches have been reported. However, most of these methods have the disadvantage [...]

Boolean Networks (BN) Based Molecular Biology Modeling: Protein Process

Yeol J.W., Barjis I., Barjis J., Barjis I., Barjis J., Berri S., Ryu Y., Polytechnic University, US

In this paper, we introduce a new application of modeling tool; Boolean Networks (BN). In particular, it shows how, by means of a Boolean Networks (BN), the protein production process can be modeled and analyzed. [...]

Imaging Ellipsometry: A Powerful Method for Biochip Development, QC and Evaluation

Laging M., Hoenig D., Eing A., Accurion, US

Biochip applications nowadays are taking the step from DNA to protein applications. Proteins show a vasting variety concerning their individual chemical behaviour, demanding new techniques regarding chip substrates and immobilization chemistries. Furthermore the use fluorescent [...]

Binding Efficiency Improvement of Antibody to Antigen on Mixed Self-Assembly Monolayers

Wu C-L, Lin Y-W, Hsu Y-L, Tseng F-G, Chieng C-C, National Tsing Hua University, TW

We make a mixed Self Assembly Monolayer for improve binding efficiency antibody, this method provide s stereo advantage and didn't need to modify antibody by moelcuar biology ono by one.

Protein Array Fabricated by Micro-Contact Printing Method for Detection of Marker Protein to Genetically Modified Organisms

Lee W., Paek S-H, Choi J.W., Sogang Univerisity, KR

For the past decade, microarray technology has evolved from genomics to proteomics. This trend offers several advantages. In small volumes, biochemical reactions may not be diffusion-limited and may thus be more efficient; less reagent and [...]

Self-Assembly of Gold Nanoparticles on E-Beam Nano-Patterns Towards Protein Nanoarray

Powell T., Yoon J-Y, University of Arizona, US

Self-assembly of gold nanoparticles on e-beam nano-patterns towards protein nanoarray. A gold colloid suspensions containing different size nanoparticles, will be dumped in succesion, and the particles will self assemble into proper sized trenches.

Ultramicro Protein Arrays for Cytokine Detection

Radke K., Ryckman K., Nettikadan S., Lynch M., Mosher C., Xu J., Henderson E., BioForce Nanosciences, Inc., US

Several solid phase microarray based methods for the detection of the genomic materials have been developed based on amplifying the target molecules. Since proteins cannot be amplified, a similar approach to protein detection faces some [...]