The liposomes were prepared using the conventional thin film hydration method with and without peptide RF-482. The loading of RF-482 was determined using BCA assay and the RSV inhibition was studied using immune-fluorescence imaging, plaque assay and PCR. There was little size change observed after loading RF-482. However, this change was not significant. It was observed that 77.3 % (n=3, ±2.2 %) protein was found non-encapsulated, hence it can be concluded that 22.7 % of peptide RF-482 was encapsulated. Plaque assay confirms above 60 % inhibition with just peptide RF-482 and liposomes. However, with peptide loaded liposomes more than 70 % inhibition was observed. Screening of RSV-F gene amplicon and comparison of viral gene amplicon and peptide, liposomes as well as functionalised liposomes along with water as negative control confirms the competitive inhibition of RSV by RF-482 as well as liposomes with and without peptide. Overall, it was found that similar to GNPs and FGNPs (Tiwari et al., 2014), empty liposomes as well as liposomes loaded with RF-482 can inhibit RSV fusion to HEP-2 cells and thereby save HEP-2 cells from infection. Immunofluorescence imaging confirms the inhibitory effect of liposomes with and without peptide RF-482.
Journal: TechConnect Briefs
Volume: 3, Biotech, Biomaterials and Biomedical: TechConnect Briefs 2017
Published: May 14, 2017
Pages: 75 - 78
Industry sector: Medical & Biotech
Topics: Biomaterials, Materials for Drug & Gene Delivery