NIH-3T3 cells were grown on nanodot arrays to investigate how cells respond to nanolandscape. The nanodot arrays consisted nanodot of diameters ranging from 10 to 200 nm which were fabricated by AAO processing on Ta-coated wafers. Cells seeded on flat wafer surface and on 10 nm nanodot array appeared normal growth. Abnormal morphology occurred to cells cultured on arrays with dot size larger than 50 nm. The abnormality turned out to be apoptosis which was validated by casepase activity assay. Coating of BSA did not affect the ability of 100 nm dot array to promote apoptosis; while coating of fibronectin or type I collagen rescued the nanotopography-induced programmed cell death. Supplementation of cytochalasin D, an IP3-K inhibitor, to cells grown on 100 nm arrays triggered early apoptosis but did not enhance proportion of cells undergoing programmed cell death. This result indicated that nanotopography-induced apoptosis shared IP3 kinase pathway. Nanotopograpgy controls life and death of cells.
Journal: TechConnect Briefs
Volume: 2, Technical Proceedings of the 2007 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: May 20, 2007
Pages: 729 - 732
Industry sector: Medical & Biotech