Chen E.T., Thornton J.T., Duh S-H
Advanced Biomimetic Sensors, Inc., US
Keywords: antibiotics, dosage kinetics, inhibition of ABeta refolding, memristive sensor, MMP2/HSP60 network, reversible membrane potential
Moonlighting proteins, such as Heat Shock Protein (HSP) and Matrix Metalloproteinase (MMP) protein, played roles in human diseases. We developed an HSP60 memristive device and an HSP/MMP-2 superconductive device for assessing a protein, ABeta refolding intrinsic energy landscape change by monitoring the antibiotic drug moxifloxacin (MOX)’s real- time effect on such change at different dosages. Sensor 1 was developed by cross-linking HSP60 with conductive polymers on gold chips. Sensor 2 was fabricated by self-assembling the HSP60 polymer mixtures on the top of an innate MMP- 2/copolymer membrane. Results showed MOX impaired ABeta’s refolding in the HSP’s cavity in real-time monitoring of the open circuit potential (OPO) change against controls. We used a Double-step chronopotentiometry (DSCPO) method to evaluate MOX’s effectiveness in promoting reversible membrane potential (RMP) values in the safe zoon for the two sensors. Sensor 2 demonstrated the ability to expel ABeta and promote normal RPM with an accuracy 97.3% and imprecision 0.05%, which was not dependent on MOX concentrations. Sensor 1 showed a lower accuracy rate, and it was dependent on MOX concentration.
Journal: TechConnect Briefs
Volume: TechConnect Briefs 2021
Published: October 18, 2021
Pages: 203 - 206
Industry sector: Sensors, MEMS, Electronics
Topics: Nanoelectronics, Sensors - Chemical, Physical & Bio
ISBN: 978-0-578-99550-2