Moxifloxacin Inhibited b-Amyloid Bio-communication and Impairs Aβ Folding with HSP60 Memristive Devices For Promoting Cell Normal Reversible Membrane Potential

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Moonlighting proteins, such as Heat Shock Protein (HSP) and Matrix Metalloproteinase (MMP) protein, played roles in human diseases. We developed an HSP60 memristive device and an HSP/MMP-2 superconductive device for assessing a protein, ABeta refolding intrinsic energy landscape change by monitoring the antibiotic drug moxifloxacin (MOX)’s real- time effect on such change at different dosages. Sensor 1 was developed by cross-linking HSP60 with conductive polymers on gold chips. Sensor 2 was fabricated by self-assembling the HSP60 polymer mixtures on the top of an innate MMP- 2/copolymer membrane. Results showed MOX impaired ABeta’s refolding in the HSP’s cavity in real-time monitoring of the open circuit potential (OPO) change against controls. We used a Double-step chronopotentiometry (DSCPO) method to evaluate MOX’s effectiveness in promoting reversible membrane potential (RMP) values in the safe zoon for the two sensors. Sensor 2 demonstrated the ability to expel ABeta and promote normal RPM with an accuracy 97.3% and imprecision 0.05%, which was not dependent on MOX concentrations. Sensor 1 showed a lower accuracy rate, and it was dependent on MOX concentration.

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Journal: TechConnect Briefs
Volume: TechConnect Briefs 2021
Published: October 18, 2021
Pages: 203 - 206
Industry sector: Sensors, MEMS, Electronics
Topics: Nanoelectronics, Sensors - Chemical, Physical & Bio
ISBN: 978-0-578-99550-2