Macromolecules in Microdevices: Multiscale simulation of DNA dynamics in model microfluidic geometries

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Simple arguments predict that the dynamics of a dissolved macromolecule confined to a channel comparable to its equilibrium coil size (~1 um for viral DNA) are quite different from those in free solution, because of the no-slip boundary condition on the fluid motion. Nevertheless, detalied, predictive computations have not been previously performed. We have incorporated a Brownian dynamics model of DNA into a fully self-consistent computational scheme that simultaneoulsy resolves the macromolecular and fluid (i.e. solvent) dynamics of DNA in a microfluidic channel. The key novel feature of this scheme is the numerical computation of the Green’s function for the flow problem, enabling a stochastic solution method that incorporates detailed hydrodynamics and respects the fluctuation-dissipation theorem. With this methodology we study a number of important confinement effects, focusing here on the retardation of relaxation of a chain in small channel.

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Journal: TechConnect Briefs
Volume: 1, Technical Proceedings of the 2002 International Conference on Modeling and Simulation of Microsystems
Published: April 22, 2002
Pages: 88 - 91
Industry sector: Sensors, MEMS, Electronics
Topic: Micro & Bio Fluidics, Lab-on-Chip
ISBN: 0-9708275-7-1