Jayanna P.K., Deinnocentes P., Bird R.C., Petrenko V.A.
Auburn University, US
Keywords: drug delivery, Landscape phage, prostate cancer, targeting
Cancer chemotherapy is complicated by a lack of selective drug targets. The use of liposomes as vectors for drug delivery has provided a route to selective accumulation of nanoparticles in the tumor interstitium due to EPR effect. To further enhance tumor chemotoxicity of liposomal drugs, they have to be precisely targeted to tumor receptors using tumor-specific probes. Herein, we describe the use of landscape phage libraries for deriving specific phage probes for PC3 prostate tumor cells. In our selection protocol, we used an extensive depletion regimen to rid the parent library of non-specific binders followed by four rounds of selection for each library. Phage probes bound to the surface were collected by elution with acid buffer (Eluate fraction) whereas the phage probes internalizing into the cells were collected followed by lysis of cells (Lysate fraction). The selected clones will be tested for their target binding efficiency in cell binding assays. Positive clones will be further tested for selectivity to target cells by comparing relative binding efficiencies to human skin fibroblasts, hepatocytes and hepatocarcinoma cells. The probes identified by these protocols will be studied as potential targeting ligands for liposomes and other nano-particulate drug vehicles.
Journal: TechConnect Briefs
Volume: 2, Nanotechnology 2008: Life Sciences, Medicine & Bio Materials – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 2
Published: June 1, 2008
Pages: 457 - 460
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topic: Biomaterials
ISBN: 978-1-4200-8504-4