In vivo nanotoxicology: Toxicoproteomics

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Proteomics applied to nanotechnology is now emerging as an attractive tool to address the still unanswered questions dealing with nano-biointeractions and NPs-induced toxicity in living systems. The unique advantage provided by “omic” techniques (such as, 2D-DIGE, LC-MS) is to get information on cellular signalling pathways as well as on specific molecular mechanisms induced by and related to NPs and/or their physical-chemical motifs. In this work, we aim at developing a proteomic-based strategy to provide insights on the impact of NPs in vivo. In particular, the ADME parameters and fate of NPs as well as the analysis of protein biomarkers were further investigated. We set up a method to obtain whole protein mapping of blood and liver of rats treated with different classes of NPs (SiO2 and QDs NPs) using 2D-DIGE and Maldi/TOF-TOF. Analysis of the protein expression profiles of the treated tissues with respect to controls were found to be altered by NPs treatments in blood and liver, activating specific biochemical pathways of cellular toxicity. Such variations were strongest after 24 h of exposure, suggesting an early acute response. Nonetheless, no general physiological damage was observed in the rats during NP exposure (in agreement with recent reports).

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2013: Bio Sensors, Instruments, Medical, Environment and Energy (Volume 3)
Published: May 12, 2013
Pages: 151 - 154
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topics: Biomaterials
ISBN: 978-1-4822-0586-2