Hyperselective and Targeted Separation of Analytes Using Multiple Dynamic Equilibrium Gradients Approach

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There is a growing need for compact and reliable advanced microfluidic devices and/or adapters for conventional capillary electrophoresis (CE) that can provide an improved resolving power of analytes.  It is demonstrated that separation of analytes can be significantly enhanced by introducing a new dynamic multiple equilibrium gradients (DMEG) approach for concentrating and separating analytes in complex mixtures with enhanced peak capacity and resolution.  It is shown that low-voltages applied as the running waveforms to specifically designed grids of electrodes microfabricated along the separation channel (or thin capillary) can increase peak capacity and separation of molecules in complex mixtures.  The running waveforms (applied to the electrodes) create moving electric field gradients, which results in very efficient separation and transport of analytes to the detection area. It is anticipated that this novel methodology will provide an unprecedented level of focusing and improved separation of analytes with similar electrophoretic mobilities in complex biological fluids.  Resolving power, focusing phenomenon, and possible future applications will be discussed. 

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Journal: TechConnect Briefs
Volume: 1, Technical Proceedings of the 2005 NSTI Nanotechnology Conference and Trade Show, Volume 1
Published: May 8, 2005
Pages: 640 - 643
Industry sector: Sensors, MEMS, Electronics
Topic: Micro & Bio Fluidics, Lab-on-Chip
ISBN: 0-9767985-0-6