Fabrication of plastic optical label-free biosensor and Immunodetection of cardiac marker in human serum

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We have studied for lab-on-a-chip (LOC) based bio-devices and fabricated plastic optical label-free biosensor device. Optical resonant reflection biosensor (ORRB) is a kind of label-free sensor, containing nanoscale resonate grating patterns1 and whole blood filter. This plastic ORRB sensor is fabricated with Cyclic Olefin Copolymer (COC) via conventional injection molding method. Injected ORRB chips employ a top plate and a bottom plate. The top plate incorporates a microfluidic channel for humam serum and the bottom plate has a sensing region, which consists of a nano-pattern, and a fluidic velocity control region referred to as a time-gate. The sensing region is formed by deposition of a high refractive index layer, Si3N4, by a plasma enhanced chemical vapor deposition (PECVD) technique. After the ORRB chip is treated by oxygen plasma in order to render it hydrophilic, the top and bottom plates are bonded by ultra sonic equipment without application of any glue. In this paper, we mention the properties of injection molding plastic ORRB sensor chip with pretreatment process, which are Si3N4, deposition conditions, immobilized process condition on plastic ORRB sensor and also experimental immunodetection results of cardiac markers in human serum. We characterized peak wavelength shifting cause of difference of optical density and refractive index from antigen-antibody interactions.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 21, 2010
Pages: 91 - 94
Industry sectors: Medical & Biotech | Sensors, MEMS, Electronics
Topicss: Chemical, Physical & Bio-Sensors, Diagnostics & Bioimaging
ISBN: 978-1-4398-3415-2