Fabrication of biosensor arrays by DPN and multiple target detection by triple wavelength fast SERRS mapping

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We have used Dip-Pen Nanolithography (DPN) to create biosensor arrays on micro- and nano-structured surfaces. Bespoke linker and labelling materials enables detection of biological molecules by surface enhanced resonance Raman scattering (SERRS). Careful DPN-directed placement of the biological species or capture chemistry, within the array, facilitates rapid read out via ultra fast Raman line mapping. Writing DPN features that complement the spectroscopic collection geometry allows the lateral resolution and detection speed to be optimised. We have recently-demonstrated massively parallel DPN, capable of covering areas > 1 cm2, and the geometry of these MEMS-generated DPN arrays can be tailored to the form factor of detecting substrates. This flexibility is key to enhancing the throughput of this combined technique by many orders of magnitude. Applied in a DPN array format, we show that SERRS offers several advantages over conventional fluorescence detection. The information rich nature of the SERRS spectrum allows multiple levels of detection capability to be embedded into each pixel, further increasing the information depth of the array. Effective practical application of multiple target detection by SERRS in a plasmonic array format is demonstrated, using suitable dye labels and resonant wavelengths of excitation.

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Journal: TechConnect Briefs
Volume: 1, Nanotechnology 2008: Materials, Fabrication, Particles, and Characterization – Technical Proceedings of the 2008 NSTI Nanotechnology Conference and Trade Show, Volume 1
Published: June 1, 2008
Pages: 509 - 512
Industry sector: Advanced Materials & Manufacturing
Topicss: Advanced Manufacturing, Nanoelectronics
ISBN: 978-1-4200-8503-7