Biological effects of PLA-TCP nanocomposites on osteoblastic cells

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Composite substrates are considered as prospective matrix to populate and support cells in the bioengineering field. Synthetic degradable biomaterials, including poly(lactic acid) (PLA) and tricalcium phosphate (TCP), are widely used for matrix scaffold. In present study, we produced nanocomposites blending PLA and TCP with various ratio. For this, PLA was dissolved in an organic solvent, chloroform and then adding TCP with ethanol. Composite structures were successfully generated at all compositions. The osteoblastic cells (MC3T3-E1) attached and spread well on PLA-TCP blending nanocomposites. In particular, the cellular growth rate and alkaline phosphatase activity was significantly higher on the PLA-TCP composites than on the pure PLA (P <0.05). Moreover, real-time gene expression of osteocalcin was prominent in PLA-TCP composite compared to the PLA. When the TCP ratio increased, mechanical strength showed parabolic appearance. These results showed us that PLA-TCP blending polymeric nanocomposites are considered to be useful as bone cell supporting matrix for the tissue regeneration.

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Journal: TechConnect Briefs
Volume: 3, Nanotechnology 2011: Bio Sensors, Instruments, Medical, Environment and Energy
Published: June 13, 2011
Pages: 233 - 235
Industry sectors: Advanced Materials & Manufacturing | Medical & Biotech
Topic: Biomaterials
ISBN: 978-1-4398-7138-6